婆罗门参丸（一种阿育吠陀多草药配方制剂）的高效液相色谱分析与标准化
HPLC analysis and standardization of Brahmi vati - An Ayurvedic poly-herbal formulation.
作者信息
Mishra Amrita, Mishra Arun K, Tiwari Om Prakash, Jha Shivesh
机构信息
Department of Pharmacognosy, School of Pharmaceutical Sciences, IFTM University, Moradabad 244001, India ; Department of Pharmaceutical Sciences, Birla Institute of Technology, Ranchi 835215, India.
Department of Pharmacognosy, School of Pharmaceutical Sciences, IFTM University, Moradabad 244001, India.
出版信息
J Young Pharm. 2013 Sep;5(3):77-82. doi: 10.1016/j.jyp.2013.09.001. Epub 2013 Sep 30.
OBJECTIVES
The aim of the present study was to standardize Brahmi vati (BV) by simultaneous quantitative estimation of Bacoside A3 and Piperine adopting HPLC-UV method. BV very important Ayurvedic polyherbo formulation used to treat epilepsy and mental disorders containing thirty eight ingredients including Bacopa monnieri L. and Piper longum L.
MATERIALS AND METHODS
An HPLC-UV method was developed for the standardization of BV in light of simultaneous quantitative estimation of Bacoside A3 and Piperine, the major constituents of B. monnieri L. and P. longum L. respectively. The developed method was validated on parameters including linearity, precision, accuracy and robustness.
RESULTS
The HPLC analysis showed significant increase in amount of Bacoside A3 and Piperine in the in-house sample of BV when compared with all three different marketed samples of the same. Results showed variations in the amount of Bacoside A3 and Piperine in different samples which indicate non-uniformity in their quality which will lead to difference in their therapeutic effects.
CONCLUSION
The outcome of the present investigation underlines the importance of standardization of Ayurvedic formulations. The developed method may be further used to standardize other samples of BV or other formulations containing Bacoside A3 and Piperine.
目的
本研究的目的是采用高效液相色谱 - 紫外检测法同时定量测定胡黄连苷A3和胡椒碱，从而对婆罗门丸（BV）进行标准化。BV是一种非常重要的阿育吠陀多草药配方，用于治疗癫痫和精神障碍，含有38种成分，包括印度假马齿苋和荜茇。
材料与方法
鉴于分别对印度假马齿苋和荜茇的主要成分胡黄连苷A3和胡椒碱进行同时定量测定，开发了一种高效液相色谱 - 紫外检测法用于BV的标准化。所开发的方法在包括线性、精密度、准确度和稳健性等参数方面进行了验证。
结果
与三种不同市售的BV样品相比，高效液相色谱分析显示自制BV样品中胡黄连苷A3和胡椒碱的含量显著增加。结果表明不同样品中胡黄连苷A3和胡椒碱的含量存在差异，这表明其质量不均匀，会导致治疗效果不同。
结论
本研究结果强调了阿育吠陀配方标准化的重要性。所开发的方法可进一步用于对BV的其他样品或含有胡黄连苷A3和胡椒碱的其他配方进行标准化。
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