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反相高效液相色谱法和紫外分光光度法同时测定胶囊中阿莫西林和氯唑西林的比较研究

Comparative Study of RP-HPLC and UV Spectrophotometric Techniques for the Simultaneous Determination of Amoxicillin and Cloxacillin in Capsules.

作者信息

Giang Do T, Hoang Vu D

机构信息

Department of Analytical Chemistry and Toxicology, Hanoi University of Pharmacy 13-15 Le Thanh Tong, Hanoi, Vietnam.

出版信息

J Young Pharm. 2010 Apr;2(2):190-5. doi: 10.4103/0975-1483.63168.

DOI:10.4103/0975-1483.63168
PMID:21264124
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3021696/
Abstract

Reversed-phase HPLC and UV spectrophotometric techniques using water as solvent have been developed and validated for the simultaneous determination of amoxicillin and cloxacillin in capsules. For both techniques, the linearity range of 60.073x2013;140.0 µg/mL was studied. The spectrophotometric data show that non-derivative techniques, such as absorbance ratio and compensation, and ratio spectra first-order derivative could be successfully used for the co-assay of amoxicillin and cloxacillin. Based on the statistical comparison of spectrophotometric and chromatographic data, the interchangeability between HPLC and UV spectrophotometric techniques has been suggested for the routine analysis.

摘要

已开发并验证了以水为溶剂的反相高效液相色谱法和紫外分光光度法，用于同时测定胶囊中的阿莫西林和氯唑西林。对于这两种技术，研究了60.073×2013；140.0μg/mL的线性范围。分光光度数据表明，非导数技术，如吸光度比和补偿以及比率光谱一阶导数，可成功用于阿莫西林和氯唑西林的联合测定。基于分光光度法和色谱数据的统计比较，建议在常规分析中HPLC和紫外分光光度法可相互替代。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/187f/3021696/8247f79e1ca4/JYPharm-2-190-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/187f/3021696/801a0312cf74/JYPharm-2-190-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/187f/3021696/3b5f13a86ffb/JYPharm-2-190-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/187f/3021696/4ca092b54a0c/JYPharm-2-190-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/187f/3021696/ed0cdfbf5309/JYPharm-2-190-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/187f/3021696/f8af4ddb4c8c/JYPharm-2-190-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/187f/3021696/8247f79e1ca4/JYPharm-2-190-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/187f/3021696/801a0312cf74/JYPharm-2-190-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/187f/3021696/3b5f13a86ffb/JYPharm-2-190-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/187f/3021696/4ca092b54a0c/JYPharm-2-190-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/187f/3021696/ed0cdfbf5309/JYPharm-2-190-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/187f/3021696/f8af4ddb4c8c/JYPharm-2-190-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/187f/3021696/8247f79e1ca4/JYPharm-2-190-g006.jpg

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