在不同时间间隔用牙胶/AH26和Resilon/RealSeal充填根管后，强力霉素吸附在根管牙本质上的稳定性。

Stability of Doxycycline Absorbed on Root Canal Dentin After Obturation with Gutta-Percha/AH26 and Resilon/RealSeal at Different Time Intervals.

作者信息

Bolhari Behnam, Meraji Naghmeh, Nosrat Ali, Hassani Shokoufeh

机构信息

Dental Research Center, Department of Endodontics, school of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.

Postgraduate Student, Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.

出版信息

J Dent (Tehran). 2013 May;10(4):367-75. Epub 2013 May 31.

PMID:24396356

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3875511/

Abstract

OBJECTIVE

Eradication of microorganisms present in the root canal system is paramount for the successful outcome of root canal therapy. The purpose of this study was to compare the of doxycycline absorbed from MTAD into root canal dentin after obturation with gutta-percha/AH26 and Resilon/RealSeal at different time intervals.

MATERIALS AND METHODS

Fifty-one extracted human teeth were instrumented. Thirty samples were obturated with either gutta-percha/AH26 or Resilon/self-etch RealSeal after final irrigation with MTAD. Fifteen samples were kept unobturated (positive control); six samples were obturated with either gutta-percha/AH26 or Resilon/self-etch RealSeal without MTAD irrigation (negative control). After aging for 1, 3 or 6 weeks, dentin debri were collected, the Doxycycline compound was extracted and its amount was quantified using high performance liquid chromatography. The statistical significance of the change in Doxycycline concentrations was tested with two-way ANOVA.

RESULTS

The mean concentration of Doxycycline in dentin for one, three and six-week guttapercha/AH26 samples was 1.8±0.36, 1.22±0.22 and 0.67±0.11 respectively, whereas these concentrations in Resilon/RealSeal samples were 1.60±0.26, 0.80±0.14 and 0.59±0.01 respectively. Regarding the positive control group, these concentrations were 2.09±0.11, 1.54±0.12 and 0.72±0.07 respectively for 1, 3 and 6-week intervals. No Doxycycline was detected in negative control groups. The Doxycycline concentrations showed a significant difference forobturating materials (p=0.008). These concentrations were higher in the gutta-percha/AH26 samples than Resilon/RealSeal samples in each time interval.

CONCLUSION

The remaining amount of Doxycycline bonded to dentin was higher when root canals were obturated with gutta-percha/AH26 compared to Resilon/RealSeal. The stability of Doxycycline showed a time dependent decrease.

摘要

目的

根除根管系统中的微生物对于根管治疗的成功结果至关重要。本研究的目的是比较在不同时间间隔用牙胶/AH26和Resilon/RealSeal充填后，MTAD中吸收的强力霉素进入根管牙本质的情况。

材料与方法

对51颗拔除的人牙进行预备。30个样本在最后用MTAD冲洗后，用牙胶/AH26或Resilon/自酸蚀RealSeal充填。15个样本不做充填（阳性对照）；6个样本用牙胶/AH26或Resilon/自酸蚀RealSeal充填但不进行MTAD冲洗（阴性对照）。在老化1、3或6周后，收集牙本质碎屑，提取强力霉素化合物，并使用高效液相色谱法对其含量进行定量。用双向方差分析检验强力霉素浓度变化的统计学意义。

结果

牙胶/AH26样本在1周、3周和6周时牙本质中强力霉素的平均浓度分别为1.8±0.36、1.22±0.22和0.67±0.11，而Resilon/RealSeal样本中的这些浓度分别为1.60±0.26、0.80±0.14和0.59±0.01。对于阳性对照组，在1周、3周和6周间隔时，这些浓度分别为2.09±0.11、1.54±0.12和0.72±0.07。阴性对照组未检测到强力霉素。强力霉素浓度在充填材料方面显示出显著差异（p = 0.008）。在每个时间间隔，牙胶/AH26样本中的这些浓度均高于Resilon/RealSeal样本。