生物纯MTAD对八株粪肠球菌的抗菌作用：一项体外研究。

The antimicrobial effect of biopure MTAD on eight strains of Enterococcus faecalis: an in vitro investigation.

作者信息

Newberry Bradley M, Shabahang Shahrokh, Johnson Neal, Aprecio Raydolfo M, Torabinejad Mahmoud

机构信息

School of Dentistry, Loma Linda University, Loma Linda, California 92350, USA.

出版信息

J Endod. 2007 Nov;33(11):1352-4. doi: 10.1016/j.joen.2007.07.006.

DOI:10.1016/j.joen.2007.07.006

PMID:17963962

Abstract

The purpose of this investigation was to determine the antimicrobial effect of MTAD as a final irrigant on eight strains of Enterococcus faecalis (E. faecalis) and to measure the minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) of MTAD. The roots of 240 extracted human teeth were instrumented using 1.3% NaOCl and 17% EDTA. The roots were divided into eight groups and contaminated with one of eight strains of E. faecalis. After irrigating with 1.3% NaOCl, the root canal and the external surfaces were exposed to MTAD for 5 minutes. Roots or dentin shavings were cultured to determine the growth of E. faecalis. The results showed that this treatment regimen was effective in completely eliminating growth in seven of eight strains of E. faecalis. The MIC/MLC tests showed that MTAD inhibited most strains of E. faecalis growth when diluted 1:8192 times and killed most strains of E. faecalis when diluted 1:512 times.

摘要

本研究的目的是确定MTAD作为最终冲洗剂对八株粪肠球菌的抗菌效果，并测定MTAD的最低抑菌浓度（MIC）和最低致死浓度（MLC）。使用1.3%次氯酸钠（NaOCl）和17%乙二胺四乙酸（EDTA）对240颗拔除的人牙进行根管预备。将牙根分为八组，分别感染八株粪肠球菌中的一株。在用1.3% NaOCl冲洗后，将根管和外表面暴露于MTAD中5分钟。对牙根或牙本质碎屑进行培养以确定粪肠球菌的生长情况。结果表明，该治疗方案能有效完全消除八株粪肠球菌中七株的生长。MIC/MLC测试表明，MTAD稀释1:8192倍时可抑制大多数粪肠球菌菌株的生长，稀释1:512倍时可杀死大多数粪肠球菌菌株。