MRI-MRS Centre, Department of Chemistry, Indian Institute of Technology-Madras, Chennai 600036, Tamil Nadu, India.
MRI-MRS Centre, Department of Chemistry, Indian Institute of Technology-Madras, Chennai 600036, Tamil Nadu, India.
J Magn Reson. 2014 Feb;239:69-74. doi: 10.1016/j.jmr.2013.12.001. Epub 2013 Dec 19.
Two dimensional homonuclear (1)H correlation spectroscopy is of considerable interest for volume localized spectral studies, both in vivo and in vitro, of biological as well as material objects. The information principally sought from correlation spectra resides in the cross-peaks, which are often masked however by the presence of diagonal peaks in COSY, or 'pseudo-diagonal' peaks at F1=0 in SECSY. It has therefore been a concern to suppress these diagonal or 'pseudo-diagonal' peaks, in order to ensure that cross-peak information is fully discernible. We present here a report of our work on volume localized DIagonal Suppressed Spin Echo Correlation specTroscopy (LDISSECT) and demonstrate its performance in comparison to the standard volume localized SECSY experiment, employing brain metabolite phantoms in a gel. The sequence works in the inhomogeneous, multi-component environment by exploiting the short acquisition time to suppress undesired information by employing an additional rf pulse. A brief description of the pulse sequence, its theory, and simulations are also included, besides experimental benchmarking on two brain metabolite phantoms in gel phase.
二维同核（1）H 相关光谱在体内和体外的生物和物质物体的容积局部光谱研究中具有相当大的兴趣。从相关光谱中主要寻求的信息存在于交叉峰中，然而，这些交叉峰常常被 COSY 中的对角峰或 SECSY 中 F1=0 处的“伪对角”峰所掩盖。因此，人们一直关注抑制这些对角或“伪对角”峰，以确保完全辨别交叉峰信息。我们在这里报告了我们在容积局部对角抑制的自旋回波相关光谱学（LDISSECT）方面的工作，并将其与标准的容积局部 SECSY 实验进行了比较，该实验在凝胶中使用脑代谢物幻影进行了演示。该序列在不均匀的多组分环境中工作，通过利用短采集时间通过施加额外的 rf 脉冲来抑制不需要的信息。除了在凝胶相中的两个脑代谢物幻影上进行实验基准测试之外，还包括脉冲序列的简要描述、其理论和模拟。
