使用 SERS 微光谱和水稳定化 Au-Ag 纳米壳在直接点印迹分析中检测皮克级细胞因子。
Femtogram detection of cytokines in a direct dot-blot assay using SERS microspectroscopy and hydrophilically stabilized Au-Ag nanoshells.
机构信息
Faculty of Chemistry and Center for Nanointegration Duisburg-Essen (CENIDE), University of Duisburg-Essen, Universitaetsstr. 5, D-45141 Essen, Germany.
出版信息
Chem Commun (Camb). 2014 Mar 14;50(21):2711-4. doi: 10.1039/c3cc48633h.
PMID:24398564
Abstract
Rapid parallel detection of two cytokines (IL-6 and IL-8) with femtogram sensitivity in a simple direct dot-blot assay is demonstrated. The microspectroscopic SERS acquisition scheme employs rationally designed, hydrophilically stabilized Au-Ag nanoshells as SERS labels, which are optimized for signal enhancement upon red laser excitation.
摘要
本文展示了一种简单的直接点印迹分析方法，可实现对两种细胞因子（IL-6 和 IL-8）进行纳升级灵敏度的快速平行检测。该微光谱 SERS 采集方案采用了经过合理设计的、亲水性稳定的 Au-Ag 纳米壳作为 SERS 标记物，这些标记物在红光激光激发下具有最佳的信号增强效果。
Zotero 插件