Ruiz-Cabello F, López Nevot M A, Garrido A, Garrido F
Nat Immun Cell Growth Regul. 1987;6(2):99-108.
We have produced a monoclonal antibody, GRM1, against a prolymphocytic leukemia that defines an antigen present in neutrophilic granulocytes (PMN) and a lymphocyte subset with natural killer (NK) activity, which was identified as large granular lymphocytes. This monoclonal antibody recognizes FcR2 (CD16), an antigen composed of two polypeptides of 50 and 60 kilodaltons, respectively. This GRM1 monoclonal antibody was tested against normal T and B cells, neutrophilic granulocytes, monocytes, platelets, acute and chronic leukemias, and was positive only against granulocytes (95%) and cells with NK activity. GRM1 was able to deplete NK cell activity in complement-dependent lysis. However, GRM1 did not block NK activity nor peripheral blood lymphocyte- and PMN-mediated antibody-dependent cytotoxicity in healthy individuals. GRM1 also did not block Fc receptor in an erythrocyte antibody rosette assay. The immunochemical data and cell distribution patterns lead us to conclude that GRM1 recognizes and FcR2 receptor epitope which is not involved in the receptor's function.
我们制备了一种针对幼淋巴细胞白血病的单克隆抗体GRM1，它可识别存在于中性粒细胞（PMN）以及具有自然杀伤（NK）活性的淋巴细胞亚群（已鉴定为大颗粒淋巴细胞）中的一种抗原。这种单克隆抗体识别FcR2（CD16），该抗原分别由两条分子量为50和60千道尔顿的多肽组成。用GRM1单克隆抗体检测了正常T细胞、B细胞、中性粒细胞、单核细胞、血小板、急性和慢性白血病，结果显示仅对粒细胞（95%）和具有NK活性的细胞呈阳性。GRM1在补体依赖性裂解中能够消耗NK细胞活性。然而，GRM1在健康个体中并不阻断NK活性，也不阻断外周血淋巴细胞和PMN介导的抗体依赖性细胞毒性。在红细胞抗体玫瑰花结试验中，GRM1也不阻断Fc受体。免疫化学数据和细胞分布模式使我们得出结论，GRM1识别的FcR2受体表位不参与该受体的功能。
