Appropriate conditions to record activation of fast Ca2+ channels in frog skeletal muscle (Rana pipiens).

Frog skeletal muscle fibres possess slow Ca++ channels which are currently studied. Only recently a fast Ca++ current in frog skeletal muscle was reported. This finding is related to the fact that this current is very labile. Here we report the appropriate conditions to record the fast calcium current (ICa-f) in skeletal muscle of the frog Rana pipiens, in isotonic solutions and resting fibre length.