Zhang Jinjing, Du Yingxiang, Zhang Qi, Lei Yuntao
Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, PR China.
Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, PR China; Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing 210009, PR China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China.
Talanta. 2014 Feb;119:193-201. doi: 10.1016/j.talanta.2013.10.042. Epub 2013 Nov 11.
Recently, chiral ionic liquids (ILs) have drawn increasing attention in chiral separation field; however, few papers reported the application of chiral ILs for chiral separation by capillary electrophoresis (CE), and among the papers, chiral ILs were mainly applied as additives to β-cyclodextrin derivatives systems to establish synergistic systems. The synergistic system based on antibiotics with chiral ILs as additives has never been reported before. In this paper, two chiral ionic liquids (ILs) based on amino acid ester, L-alanine and L-valine tert butyl ester bis (trifluoromethane) sulfonamide, were first applied to evaluate the synergistic effect with antibiotic selector for CE chiral separation. The vancomycin-based synergistic system with chiral ILs as additives was successfully established and investigated for the enantioseparation of naproxen, carprofen, ibuprofen, ketoprofen and pranoprofen. Compared to vancomycin-alone cases, significant improvement of separation for all the analytes was observed. Several parameters, such as type and proportion of organic modifier, composition and pH of buffer, concentration of chiral ILs and vancomycin were systematically investigated, and then evaluated by means of Statistical Product and Service Solutions (SPSS) to research the influences on the synergistic effect. Finally, the established method was successfully applied to test the chiral impurity of naproxen sample.
近年来，手性离子液体（ILs）在手性分离领域受到越来越多的关注；然而，鲜有论文报道手性离子液体在毛细管电泳（CE）手性分离中的应用，并且在这些论文中，手性离子液体主要作为添加剂应用于β-环糊精衍生物体系以建立协同体系。基于抗生素并以手性离子液体作为添加剂的协同体系此前从未见报道。本文首次应用两种基于氨基酸酯的手性离子液体，L-丙氨酸和L-缬氨酸叔丁酯双（三氟甲烷）磺酰胺，来评估其与抗生素选择剂在CE手性分离中的协同效应。成功建立了以手性离子液体为添加剂的基于万古霉素的协同体系，并用于萘普生、卡洛芬、布洛芬、酮洛芬和普拉洛芬的对映体分离研究。与单独使用万古霉素的情况相比，所有分析物的分离效果都有显著改善。系统研究了有机改性剂的类型和比例、缓冲液的组成和pH值、手性离子液体和万古霉素的浓度等几个参数，然后通过统计产品与服务解决方案（SPSS）进行评估，以研究其对协同效应的影响。最后，所建立的方法成功应用于萘普生样品手性杂质的检测。
