Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, PR China.
J Chromatogr A. 2013 Sep 6;1306:97-103. doi: 10.1016/j.chroma.2013.07.053. Epub 2013 Jul 19.
In this paper, two novel amino acid chiral ILs, tetramethylammonium-l-arginine (TMA-l-Arg) and tetramethylammonium-l-aspartic acid (TMA-l-Asp), were applied for the first time in CE enantioseparation to evaluate their potential synergistic effect with glycogen as chiral selector. As observed, significantly improved separation of tested enantiomers were obtained in the chiral ILs/glycogen synergistic systems compared to the single glycogen separation system. Several primary parameters affecting the enantioseparation, such as amino acid ILs (AAILs) concentration, glycogen concentration and buffer pH, were systematically investigated. An achiral tetramethylammonium hydroxide ionic liquid (TMA-OH) modified separation system was also evaluated to validate the superiority of the novel chiral ILs/glycogen synergistic systems. To further optimize the overall synergistic systems, the effect of three other parameters, including buffer concentration, applied voltage and capillary temperature were simultaneously analyzed by a central composite design (CCD), and excellent enantioseparations were achieved with the optimized parameters. The results indicate that the application of chiral ILs/glycogen synergistic systems is a promising way in chiral separation science.
本文首次将两种新型氨基酸手性离子液体，四甲基胍-l-精氨酸（TMA-l-Arg）和四甲基胍-l-天冬氨酸（TMA-l-Asp），应用于 CE 手性分离中，以评估它们与糖原为手性选择剂的潜在协同作用。结果表明，与单一糖分离体系相比，在手性离子液体/糖协同体系中，测试对映异构体的分离得到了显著改善。系统研究了影响对映体分离的几个主要参数，如氨基酸离子液体（AAILs）浓度、糖浓度和缓冲液 pH 值。还评估了一种非手性四甲基氢氧化铵离子液体（TMA-OH）修饰的分离体系，以验证新型手性离子液体/糖协同体系的优越性。为了进一步优化整体协同体系，通过中心复合设计（CCD）同时分析了三个其他参数的影响，包括缓冲液浓度、外加电压和毛细管温度，并使用优化的参数实现了出色的对映体分离。结果表明，手性离子液体/糖协同体系的应用是手性分离科学的一种有前途的方法。
