Pohanka Miroslav
Faculty of Military Health Sciences, University of Defence, Trebesska 1575, CZ-500 01 Hradec Kralove, Czech Republic; Karel English College in Brno, Sujanovo namesti 356/1, 60200 Brno, Czech Republic.
Talanta. 2014 Feb;119:412-6. doi: 10.1016/j.talanta.2013.11.045. Epub 2013 Nov 22.
Butyrylcholinesterase (BChE) is an enzyme abundantly constituted in the livers and released into blood where it is soluble. It may be found in the both plasma and serum. BChE can serve as a biochemical marker. BChE activity is typically measured by spectrophotometric Ellman's method. In the present work, voltammetric assay of cholinesterasemia is proposed as a simple and reliable method. In the experiments described here, limits of detections 4.57 pkat for the spectrofotometric test and 1.14 pkat for the voltammetric assay were determined. Interference caused by acetylcholinesterase (AChE) and organic solvents was characterized and counter measurement to the AChE caused interference was proposed. Finally, the both methods were correlated one to each other using mouse plasma spiked with carbofuran resulting in a promising coefficient of determination. In a conclusion, the voltammetric assay seems to be reliable and suitable for routine performance.
丁酰胆碱酯酶（BChE）是一种在肝脏中大量合成并释放到血液中的可溶性酶。它可存在于血浆和血清中。BChE可作为一种生化标志物。BChE活性通常采用分光光度法中的埃尔曼法进行测定。在本研究中，提出了一种用于检测血胆碱酯酶的伏安法，该方法简单且可靠。在此处描述的实验中，分光光度法的检测限为4.57皮卡特，伏安法的检测限为1.14皮卡特。对乙酰胆碱酯酶（AChE）和有机溶剂引起的干扰进行了表征，并提出了针对AChE引起的干扰的应对措施。最后，使用添加了呋喃丹的小鼠血浆将两种方法相互关联，得到了一个有前景的决定系数。总之，伏安法似乎可靠且适用于常规检测。
