Zhang Zhaodi, Zhang Lingyi, Zhang Chenggong, Zhang Weibing
Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China; Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai, PR China.
Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China.
Talanta. 2014 Feb;119:485-91. doi: 10.1016/j.talanta.2013.11.037. Epub 2013 Nov 27.
A novel enzymatic reactor was prepared by incorporating SBA-15 nanoparticles into hybrid organic-inorganic monolith and immobilizing trypsin with glutaraldehyde as bridging reagent. Preparation and operation conditions including nanoparticles percentage and residence time were optimized to improve the digestion efficiency. The digestion products were characterized by Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) with sequence coverage of 50%, 93% and 71% for bovine serum albumin, myoglobin and cytochrome C, while consuming only about 19s in dynamic mode. Compared with enzymatic reactor without nanoparticles incorporated, the enzymatic reactor with SBA-15 nanoparticles embedded achieved higher digestion efficiency by introducing more trypsin, which was originated from combination of SBA-15 nanoparticles and hybrid organic-inorganic monolith.
通过将SBA - 15纳米颗粒掺入有机 - 无机杂化整体材料中，并以戊二醛作为桥联试剂固定胰蛋白酶，制备了一种新型酶反应器。优化了包括纳米颗粒百分比和停留时间在内的制备和操作条件，以提高消化效率。采用基质辅助激光解吸电离飞行时间质谱（MALDI - TOF - MS）对消化产物进行表征，牛血清白蛋白、肌红蛋白和细胞色素C的序列覆盖率分别为50%、93%和71%，而在动态模式下仅消耗约19秒。与未掺入纳米颗粒的酶反应器相比，嵌入SBA - 15纳米颗粒的酶反应器通过引入更多的胰蛋白酶实现了更高的消化效率,这源于SBA - 15纳米颗粒与有机 - 无机杂化整体材料的结合。
