Tartabini Mirta L, Bonino Guillermo S, Racca Liliana, Luque Alicia G
Centro de Referencia de Micología (CEREMIC), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina.
Centro de Referencia de Micología (CEREMIC), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina.
Rev Argent Microbiol. 2013 Oct-Dec;45(4):248-53. doi: 10.1016/S0325-7541(13)70031-2.
Due to the pleomorphism and cultural variability displayed by species of the genus Trichophyton, the identification methods based solely on morphological features are usually insufficient for their classification. The goal of the present work was to test a set of phenotypic methods in order to identify fungal isolates that belong to the aforementioned genus. These methods were based on a molecular taxonomic technique used as standard. Clinical isolates (56) were used as samples along with 6 reference strains. Macro and micromorphological studies were performed as well as biochemical and physiological tests such as in vitro hair perforation, nutritional requirements in Trichophyton agar media, urease production and growth on bromocresol purple-milk. solids-glucose (BCP-MS-G) agar. Additionally, PCR fingerprinting using the (GACA)4 primer was employed. As a result of the PCR method, specific profiles were observed for Microsporum canis, Epidermophyton floccosum, Trichophyton rubrum and Trichophyton interdigitale. Identical profiles were obtained for Arthroderma benhamiae y Trichophyton erinacei. Of the total number of clinical isolates, 39 matched the T. rubrum profile while 13 corresponded to A. benhamiae and 4 to T. interdigitale. The most useful phenotypic test to differentiate between T. rubrum and T. mentagrophytes complex strains was alkalinization of the BCP-MS-G medium. Phenotypic tests did not allow differentiation among the T. mentagrophytes complex species. On the other hand, the molecular technique allowed characterization of T. rubrum isolates as well as of those observed in our study and included in the T. mentagrophytes complex: T. interdigitale and Trichophyton sp., the anamorph of A. benhamiae.
由于毛癣菌属物种表现出多形性和培养变异性，仅基于形态特征的鉴定方法通常不足以对其进行分类。本研究的目的是测试一组表型方法，以鉴定属于上述属的真菌分离株。这些方法基于一种用作标准的分子分类技术。使用56株临床分离株作为样本，以及6株参考菌株。进行了宏观和微观形态学研究，以及生化和生理学测试，如体外毛发穿孔试验、毛癣菌琼脂培养基中的营养需求、脲酶产生以及在溴甲酚紫-牛奶-葡萄糖（BCP-MS-G）琼脂上的生长情况。此外，还采用了使用（GACA）4引物的PCR指纹图谱分析。PCR方法的结果显示，犬小孢子菌、絮状表皮癣菌、红色毛癣菌和指间毛癣菌具有特定的图谱。棒状杆菌和须癣毛癣菌获得了相同的图谱。在临床分离株总数中，39株与红色毛癣菌图谱匹配，13株与棒状杆菌匹配，4株与指间毛癣菌匹配。区分红色毛癣菌和须癣毛癣菌复合菌株最有用的表型测试是BCP-MS-G培养基的碱化。表型测试无法区分须癣毛癣菌复合菌种。另一方面，分子技术能够对红色毛癣菌分离株以及在我们的研究中观察到并包含在须癣毛癣菌复合菌中的菌株进行表征：指间毛癣菌和棒状杆菌的无性型毛癣菌属。
