[用于检测幼虫芽孢杆菌（蜜蜂美洲幼虫腐臭病的病原体）对四环素敏感性的Epsilometer（Etest）方法的评估]
[Evaluation of the Epsilometer (Etest) method for the detection of tetracycline susceptibility in Paenibacillus larvae, the causal agent of American foulbrood disease of honeybees].
作者信息
Alippi Adriana M, Reynaldi Francisco J, López Ana C
机构信息
Centro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
Centro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
出版信息
Rev Argent Microbiol. 2013 Oct-Dec;45(4):257-61. doi: 10.1016/S0325-7541(13)70033-6.
American foulbrood (AFB) is a bacterial disease caused by the spore-forming, grampositive bacterium Paenibacillus larvae, which affects honeybee broods worldwide. The aim of this work was to compare the Epsilometer test (Etest) to the agar dilution method for testing a collection of 22 P. larvae strains to tetracycline by using MYPGP and Iso- Sensitest agars. Results showed that a categorical agreement of 100% was found when using Iso-Sensitest, while a categorical agreement of 86.36% was found (with 3 minor errors) when MYPGP was tested. In conclusion, the Etest could be a rapid and reliable method for testing MIC values of tetracycline in P. larvae only when used in combination with Iso-Sensitest agar. Nevertheless, these results should be confirmed with future studies involving a larger number of isolates.
美洲幼虫腐臭病（AFB）是一种由形成芽孢的革兰氏阳性细菌幼虫类芽孢杆菌引起的细菌性疾病，它影响着全球范围内的蜜蜂幼虫。这项工作的目的是通过使用MYPGP琼脂和Iso - Sensitest琼脂，将Epsilometer试验（Etest）与琼脂稀释法进行比较，以检测22株幼虫类芽孢杆菌对四环素的敏感性。结果表明，使用Iso - Sensitest琼脂时，分类一致性为100%，而检测MYPGP琼脂时，分类一致性为86.36%（有3个小误差）。总之，仅当Etest与Iso - Sensitest琼脂联合使用时，它才可能是一种快速且可靠的检测幼虫类芽孢杆菌对四环素最低抑菌浓度（MIC）值的方法。然而，这些结果应通过未来涉及更多分离株的研究加以证实。
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