Marketou M E, Kontaraki J, Zacharis E, Parthenakis F, Maragkoudakis S, Gavras I, Gavras H, Vardas P E
Cardiology Department, Heraklion University Hospital, Crete, Greece.
Hypertension and Atherosclerosis Section, Department of Medicine, Boston University School of Medicine, Boston, MA, USA.
J Hum Hypertens. 2014 Jul;28(7):450-5. doi: 10.1038/jhh.2013.133. Epub 2014 Jan 9.
Bradykinin participates in various hypertensive processes, exerted via its type 1 and type 2 receptors (BKR1 and BKR2). The aim of the study was to investigate BKR1 and BK2R gene expression in peripheral monocytes in patients with essential hypertension compared with healthy individuals. Seventeen hypertensive patients (9 males, age 56 ± 7 years) and 12 healthy individuals (7 males, age 55 ± 6) participated. Mononuclear cells isolated using anti-CD14+ antibodies and mRNAs of BKR1 and BKR2 were estimated by real-time quantitative reverse transcription-PCR. Both BKR1 and BKR2 showed significantly upregulated gene expression in the group of hypertensive patients. Specifically, BKR1 gene expression was 142.1 ± 42.2 in hypertensives versus 20.2 ± 8 in controls (P = 0.024) and BKR2 was 1222.2 ± 361.6 in hypertensives versus 259.5 ± 99.1 in controls (P = 0.038). Antihypertensive treatment resulted in a decrease in BKR1 (from 142.1 ± 42.2 to 55.2 ± 17.1, P = 0.065) and in BKR2 (from 1222.2 ± 361.6 to 256.8 ± 81.8, P = 0.014) gene expression. BKR1 and BKR2 gene expression on peripheral monocytes is upregulated in essential hypertension. This may lead to functional changes in monocytes and contribute to the development of target organ damage in hypertensive patients.
缓激肽通过其1型和2型受体（BKR1和BKR2）参与各种高血压过程。本研究的目的是调查原发性高血压患者与健康个体相比外周血单核细胞中BKR1和BK2R基因的表达情况。17例高血压患者（9例男性，年龄56±7岁）和12例健康个体（7例男性，年龄55±6岁）参与了研究。使用抗CD14 +抗体分离单核细胞，并通过实时定量逆转录PCR估计BKR1和BKR2的mRNA。在高血压患者组中，BKR1和BKR2均显示基因表达显著上调。具体而言，高血压患者的BKR1基因表达为142.1±42.2，而对照组为20.2±8（P = 0.024），BKR2在高血压患者中为1222.2±361.6，而对照组为259.5±99.1（P = 0.038）。抗高血压治疗导致BKR1（从142.1±42.2降至55.2±17.1，P = 0.065）和BKR2（从1222.2±361.6降至256.8±81.8，P = 0.014）基因表达降低。原发性高血压患者外周血单核细胞中BKR1和BKR2基因表达上调。这可能导致单核细胞功能改变，并有助于高血压患者靶器官损伤的发展。
