活性己糖相关化合物下调胰腺癌 HSP27 表达，并增强吉西他滨的细胞毒性作用。

Active hexose-correlated compound down-regulates HSP27 of pancreatic cancer cells, and helps the cytotoxic effect of gemcitabine.

机构信息

Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan.

出版信息

Anticancer Res. 2014 Jan;34(1):141-6.

PMID:24403454

Abstract

BACKGROUND/AIM: Active hexose-correlated compound (AHCC), an extract of basidiomycete mushroom, is used as health food to enhance the therapeutic effects and reduce the adverse effects of chemotherapy. Our previous proteomic analysis revealed that up-regulation of heat-shock protein 27 (HSP27) was responsible for gemcitabine resistance of pancreatic cancer cells. The aim of the present study was to investigate the effect of AHCC on the expression of HSP27 and the effect of combinatorial treatment of AHCC and gemcitabine on the gemcitabine-resistant pancreatic cancer cell line KLM1-R.

MATERIALS AND METHODS

KLM1-R cells were treated with AHCC, and the expression of HSP27 as well as the cytotoxic effects of combinatorial treatment of AHCC and gemcitabine were investigated with western blotting and MTS assay, respectively.

RESULTS

AHCC down-regulated HSP27 and exhibited a cytotoxic effect on KLM1-R cells. Furthermore, the cytotoxic effect of the combinatorial treatment of AHCC and gemcitabine was synergistic.

CONCLUSION

This study supports the potential therapeutic benefits of combinatorial treatment of AHCC and gemcitabine for patients with pancreatic cancer.

摘要

背景/目的：活性己糖相关化合物（AHCC）是一种从担子菌蘑菇中提取的物质，被用作保健品以增强化疗的疗效并降低其副作用。我们之前的蛋白质组学分析表明，热休克蛋白 27（HSP27）的上调导致了胰腺癌细胞对吉西他滨的耐药性。本研究的目的是探讨 AHCC 对 HSP27 表达的影响，以及 AHCC 与吉西他滨联合治疗对吉西他滨耐药的胰腺癌细胞系 KLM1-R 的影响。