Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan.
Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan
Anticancer Res. 2014 Sep;34(9):4807-11.
BACKGROUND/AIM: Active hexose-correlated compound (AHCC) is an extract of basidiomycete mushroom. It has been used as health food due to its efficacy of enhancing antitumor effects and reducing adverse effects of chemotherapy. Our previous research showed that AHCC down-regulated heat-shock protein (HSP)-27 and exhibited cytotoxic effects against gemcitabine-resistant pancreatic cancer cells. Sex-determining region Y-box 2 (SOX2) is reported to be up-regulated in other kinds of cancer cells and involved in carcinogenesis and malignancy. The aim of this study was to investigate the effects of AHCC on protein expression of SOX2 in the gemcitabine-resistant pancreatic cancer cell line KLM1-R.
AHCC was applied to KLM1-R cells and expression of SOX2 was analyzed by western blotting.
AHCC down-regulated SOX2 in KLM1-R cells. Nanog and Oct4, co-workers of SOX2 in maintaining pluripotency, did not exhibit any significant change in protein expression.
We showed the potential of AHCC to be a candidate for combinatorial therapy in anticancer drug regimens. This result suggests that the target of AHCC in expressing therapeutic efficacy was not the pluripotent cells such as cancer stem cells (CSCs) but SOX2-specific.
背景/目的：活性己糖相关化合物（AHCC）是一种担子菌菇提取物。由于其增强抗肿瘤作用和降低化疗副作用的功效，已被用作保健品。我们之前的研究表明，AHCC 下调热休克蛋白（HSP）-27 并对吉西他滨耐药胰腺癌细胞表现出细胞毒性作用。性别决定区 Y 框 2（SOX2）在其他类型的癌细胞中被报道上调，并参与致癌和恶性。本研究旨在探讨 AHCC 对吉西他滨耐药胰腺癌细胞系 KLM1-R 中 SOX2 蛋白表达的影响。
将 AHCC 应用于 KLM1-R 细胞，通过 Western 印迹分析 SOX2 的表达。
AHCC 下调 KLM1-R 细胞中的 SOX2。多能性的共同工作者 Nanog 和 Oct4 在蛋白质表达上没有明显变化。
我们展示了 AHCC 作为抗癌药物联合治疗候选药物的潜力。这一结果表明，AHCC 表达治疗功效的靶标不是多能性细胞（如癌症干细胞（CSCs）），而是 SOX2 特异性。
