Department of Measurement Technology and Industrial Electrical Engineering, Lund University, Lund, Sweden.
Department of Measurement Technology and Industrial Electrical Engineering, Lund University, Lund, Sweden ; Lund University, CREATE Health, Lund, Sweden.
Biomicrofluidics. 2013 Mar 28;7(2):24107. doi: 10.1063/1.4798473. eCollection 2013.
Acoustic trapping of minute bead amounts against fluid flow allows for easy automation of multiple assay steps, using a convenient aspirate/dispense format. Here, a method based on acoustic trapping that allows sample preparation for immuno-matrix-assisted laser desorption/ionization mass spectrometry using only half a million 2.8 μm antibody covered beads is presented. The acoustic trapping is done in 200 × 2000 μm(2) glass capillaries and provides highly efficient binding and washing conditions, as shown by complete removal of detergents and sample processing times of 5-10 min. The versatility of the method is demonstrated using an antibody against Angiotensin I (Ang I), a peptide hormone involved in hypotension. Using this model system, the acoustic trapping was efficient in enriching Angiotensin at 400 pM spiked in plasma samples.
利用声捕获技术，即使只有 50 万个 2.8μm 抗体覆盖的珠子，也可以轻松地自动化进行多个检测步骤，从而实现对微量 bead 数量的流体流动的捕获。本文介绍了一种基于声捕获的方法，仅使用半百万个 2.8μm 抗体覆盖的珠子，即可完成用于免疫基质辅助激光解吸/电离质谱法的样品制备。声捕获在 200×2000μm(2)玻璃毛细管中进行，并提供了高效的结合和洗涤条件，如完全去除洗涤剂和 5-10min 的样品处理时间所示。该方法的多功能性通过针对血管紧张素 I (Ang I)的抗体进行了演示，Ang I 是一种参与低血压的肽激素。使用该模型系统，声捕获在富集血浆样品中 400pM 浓度的血管紧张素方面非常有效。
