Walz W, Mozaffari B
Brain Res. 1987 Jun 2;412(2):405-8. doi: 10.1016/0006-8993(87)91152-8.
Barium-sensitive channel-mediated unidirectional K+ influx into mouse and rat astrocytes and its dependency upon cell density and serum was investigated. The huge difference in flux velocity between mouse (about 1000-2000 nmol X mg-1 X min-1, depending on culture conditions) and rat astrocytes (100-20 nmol X mg-1 X min-1) could not be primarily explained by an effect of culture conditions. Some small effects of both cell density and the use of fetal calf serum were, however, seen on the fluxes in mouse as well as rat astrocytes. It seems that rat astrocytes in culture lose irreversibly a glial K+ channel. An alternative is that the results may reflect a lower level of K+ channels in rat astrocytes in situ.
研究了钡敏感通道介导的钾离子单向流入小鼠和大鼠星形胶质细胞的过程及其对细胞密度和血清的依赖性。小鼠（根据培养条件，约为1000 - 2000 nmol·mg⁻¹·min⁻¹）和大鼠星形胶质细胞（100 - 20 nmol·mg⁻¹·min⁻¹）之间通量速度的巨大差异，不能主要用培养条件的影响来解释。然而，细胞密度和胎牛血清的使用对小鼠和大鼠星形胶质细胞的通量都有一些小的影响。似乎培养中的大鼠星形胶质细胞不可逆地失去了一种胶质钾离子通道。另一种可能是，这些结果可能反映了原位大鼠星形胶质细胞中钾离子通道的水平较低。
