Molecular Pathology, Institute of Biomedicine and Translational Medicine, University of Tartu, 19 Ravila St, Tartu 50411, Estonia.
Immun Ageing. 2014 Jan 9;11(1):1. doi: 10.1186/1742-4933-11-1.
Ageing affects many components of the immune system, including innate immune cells like monocytes. They are important in the early response to pathogens and for their role to differentiate into macrophages and dendritic cells. Recent studies have revealed significant age-related changes in genomic DNA methylation in peripheral blood mononuclear cells, however information on epigenetic changes in specific leukocyte subsets is still lacking. Here, we aimed to analyse DNA methylation in purified monocyte populations from young and elderly individuals.
We analysed the methylation changes in monocytes purified from young and elderly individuals using the HumanMethylation450 BeadChip array. Interestingly, we found that among 26 differentially methylated CpG sites, the majority of sites were hypomethylated in elderly individuals. The most hypomethylated CpG sites were located in neuropilin 1 (NRP1; cg24892069) and neurexin 2 (NRXN2; cg27209729) genes, and upstream of miR-29b-2 gene (cg10501210). The age-related hypomethylation of these three sites was confirmed in a separate group of young and elderly individuals.
We identified significant age-related hypomethylation in human purified monocytes at CpG sites within the regions of NRP1, NRXN2 and miR-29b-2 genes.
衰老影响免疫系统的许多组成部分，包括先天免疫细胞如单核细胞。它们在病原体的早期反应和分化为巨噬细胞和树突状细胞方面起着重要作用。最近的研究揭示了外周血单个核细胞中基因组 DNA 甲基化与年龄相关的显著变化，然而，关于特定白细胞亚群的表观遗传变化的信息仍然缺乏。在这里，我们旨在分析来自年轻人和老年人的纯化单核细胞群体中的 DNA 甲基化。
我们使用 HumanMethylation450 BeadChip 阵列分析了从年轻人和老年人中纯化的单核细胞中的甲基化变化。有趣的是，我们发现，在 26 个差异甲基化的 CpG 位点中，大多数在老年人中是低甲基化的。最明显的低甲基化 CpG 位点位于神经肽 1（NRP1；cg24892069）和神经连接蛋白 2（NRXN2；cg27209729）基因以及 miR-29b-2 基因的上游（cg10501210）。在另一组年轻人和老年人中，这三个位点的年龄相关低甲基化得到了证实。
我们在人类纯化的单核细胞中鉴定到与年龄相关的 CpG 位点的显著低甲基化，这些 CpG 位点位于 NRP1、NRXN2 和 miR-29b-2 基因区域内。
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