Mekori Y A, Chang J C
Cell Immunol. 1987 Aug;108(1):1-9. doi: 10.1016/0008-8749(87)90188-2.
The effects of mast cell activation/degranulation on the elicitation of contact sensitivity (CS) to oxazolone and dinitrofluorobenzene were investigated. Mice were actively sensitized to oxazolone by epicutaneous painting followed by ear challenge. Passive sensitization to DNFB was induced by intradermal injections of dinitrophenol (DNP)-specific cloned T cells in the ears. Mast cells in the challenged ears were activated in various time periods by inducing a passive cutaneous anaphylaxis reaction where passive sensitization with monoclonal IgE anti-DNP antibodies was followed by iv injection of DNP-BSA. This combination of immediate and delayed-type hypersensitivity reactions resulted in a significant increase of ear swelling without any noticeable effect on cellular infiltration when the contact response was evaluated a short time (3-4 hr) after mast cell activation. The very same results were obtained in naive (unsensitized) mice, indicating that this reaction was nonspecific. However, when the CS reaction was evaluated at its peak, i.e., 24 hr post challenge, mast cell activation that had been induced 0.5-11 hr after ear challenge did not have any significant effect on both swelling and cellular infiltration when the latter was evaluated by a radiometric assay. We conclude that in these systems mast cell activation/degranulation makes little or no contribution to the modulation of T-cell activity.
研究了肥大细胞活化/脱颗粒对恶唑酮和二硝基氟苯接触敏感性（CS）激发的影响。通过表皮涂抹使小鼠对恶唑酮产生主动致敏，随后进行耳部激发。通过在耳部皮内注射二硝基苯酚（DNP）特异性克隆T细胞诱导对二硝基氟苯的被动致敏。通过诱导被动皮肤过敏反应在不同时间段激活激发耳部的肥大细胞，即先用抗DNP单克隆IgE抗体进行被动致敏，然后静脉注射DNP-BSA。当在肥大细胞激活后短时间（3-4小时）评估接触反应时，这种速发型和迟发型超敏反应的组合导致耳部肿胀显著增加，而对细胞浸润没有任何明显影响。在未致敏的幼稚小鼠中也得到了完全相同的结果，表明这种反应是非特异性的。然而，当在激发后24小时即CS反应的峰值时进行评估，耳部激发后0.5-11小时诱导的肥大细胞激活对肿胀和细胞浸润均无显著影响，后者通过放射性测定法进行评估。我们得出结论，在这些系统中，肥大细胞活化/脱颗粒对T细胞活性的调节作用很小或没有作用。
