地钱悬浮培养细胞的光合自养生长。

Photoautotrophic growth of Marchantia polymorpha L. cells in suspension culture.

机构信息

Institute of Food Chemistry, Shimamoto-cho, Mishima-gun, 618, Osaka, Japan.

出版信息

Planta. 1979 Jan;144(5):509-10. doi: 10.1007/BF00380131.

Abstract

A cell line of M. polymorpha was grown photoautotrophically in liquid suspension culture using 1% CO2 in air as sole carbon source. The growth rate in terms of cell dry-weight during the exponential phase was 0.171 and the doubling time was 1.76 d. The rate of increase in chlorophyll was 1.6 times higher than the growth rate. The highest content of chlorophyll was 24 mg g(-1) dry weight, and the photosynthetic activity of the cells in the exponential phase, as calculated from the growth rate, was at least 60 μmol mg(-1) chlorophyll h(-1).

摘要

一株多形微囊藻细胞系在液体悬浮培养中以空气中 1% CO2 作为唯一碳源进行了自养生长。在指数生长期，细胞干重的生长速率为 0.171，倍增时间为 1.76 d。叶绿素的增长率比生长速率高 1.6 倍。叶绿素的最高含量为 24mg g(-1)干重，根据生长速率计算得出，指数生长期细胞的光合活性至少为 60 μmol mg(-1)叶绿素 h(-1)。