Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan,
Appl Microbiol Biotechnol. 2014 Mar;98(5):1963-70. doi: 10.1007/s00253-013-5474-9. Epub 2014 Jan 10.
Virus-like particles (VLPs) can be produced in recombinant protein production systems by expressing viral surface proteins that spontaneously assemble into particulate structures similar to authentic viral or subviral particles. VLPs serve as excellent platforms for the development of safe and effective vaccines and diagnostic antigens. Among various recombinant protein production systems, the baculovirus-insect cell system has been used extensively for the production of a wide variety of VLPs. This system is already employed for the manufacture of a licensed human papillomavirus-like particle vaccine. However, the baculovirus-insect cell system has several inherent limitations including contamination of VLPs with progeny baculovirus particles. Stably transformed insect cells have emerged as attractive alternatives to the baculovirus-insect cell system. Different types of VLPs, with or without an envelope and composed of either single or multiple structural proteins, have been produced in stably transformed insect cells. VLPs produced by stably transformed insect cells have successfully elicited immune responses in vivo. In some cases, the yield of VLPs attained with recombinant insect cells was comparable to, or higher than, that obtained by baculovirus-infected insect cells. Recombinant insect cells offer a promising approach to the development and production of VLPs.
病毒样颗粒（VLPs）可以通过表达病毒表面蛋白在重组蛋白生产系统中产生，这些蛋白会自发组装成类似真实病毒或亚病毒颗粒的颗粒状结构。VLPs 是安全有效疫苗和诊断抗原开发的理想平台。在各种重组蛋白生产系统中，杆状病毒-昆虫细胞系统已被广泛用于生产各种 VLPs。该系统已被用于制造已获得许可的人类乳头瘤病毒样颗粒疫苗。然而，杆状病毒-昆虫细胞系统存在一些固有局限性，包括 VLPs 与子代杆状病毒颗粒的污染。稳定转化的昆虫细胞已成为杆状病毒-昆虫细胞系统的有吸引力的替代品。不同类型的 VLPs，有或没有包膜，由单个或多个结构蛋白组成，已在稳定转化的昆虫细胞中产生。在体内，用稳定转化的昆虫细胞产生的 VLPs 成功地引发了免疫反应。在某些情况下，用重组昆虫细胞获得的 VLPs 的产量与用杆状病毒感染的昆虫细胞获得的产量相当，甚至更高。重组昆虫细胞为 VLPs 的开发和生产提供了一种有前途的方法。
