植物 tRNA 核苷酸转移酶：I. 羽扇豆种子 tRNA 核苷酸转移酶的分离与纯化。
Plant tRNA nucleotidyltransferase : I. Isolation and purification of tRNA nucleotidyltransferase from Lupinus luteus seeds.
机构信息
Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 30/36, 60-479, Poznań, Poland.
出版信息
Planta. 1978 Jan;142(1):23-7. doi: 10.1007/BF00385115.
PMID:24407993
Abstract
A method for purification of tRNA nucleotidyltransferase from Lupinus luteus L. seeds on a preparative scale is presented. This involved ammonium sulfate fractionation and chromatography on DEAE-cellulose, hydroxylapatite, and QAE-Sephadex A-50. The final enzyme prepartion was apparently homogeneous and a 2000-fold purification was achieved. Data presented suggest the existence of only one form of tRNA nucleotidyltransferase in dry lupin seeds.
摘要
一种从羽扇豆种子中制备级纯化 tRNA 核苷酸转移酶的方法。该方法包括硫酸铵分级沉淀和 DEAE-纤维素、羟磷灰石和 QAE-葡聚糖 A-50 层析。最终的酶制剂显然是均一的，实现了 2000 倍的纯化。所提供的数据表明，在干燥的羽扇豆种子中只存在一种形式的 tRNA 核苷酸转移酶。
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