Maley B E, Newton B W, Howes K A, Herman L M, Oloff C M, Smith K C, Elde R P
J Comp Neurol. 1987 Jun 22;260(4):483-90. doi: 10.1002/cne.902600402.
The nucleus tractus solitarii in the monkey Macaca mulatta was found to have several subdivisions based upon cytoarchitectonics and immunohistochemistry. Subdivisions that could be identified included commissural, medial, parvicellular, dorsolateral, ventrolateral, intermediate, and interstitial. Substance P and enkephalin immunoreactivity was localized within discrete regions of the nucleus tractus solitarii, by means of the peroxidase-antiperoxidase technique. Substance P immunoreactivity occurred most frequently in the interstitial subdivision of the nucleus tractus solitarii. Moderate accumulations of substance P immunoreactivity were present in the commissural, medial, parvicellular, dorsolateral, and intermediate subdivisions, but very little was present in the ventrolateral subdivision. Enkephalin immunoreactivity followed the staining patterns of substance P; however, the amounts of enkephalin immunoreactivity were less than amounts for substance P. Following colchicine treatment, large numbers of enkephalin-immunoreactive neurons were distributed throughout all subdivisions, many being located in the parvicellular and medial subdivisions. The few substance P-immunoreactive neurons found were restricted to the parvicellular subdivision. The distribution of substance P and enkephalin immunoreactivity in M. mulatta is very similar to that described in the cat and rat. In addition, the extensive overlap of the distribution of these two putative neurotransmitters provides morphological evidence for their possible participation in the autonomic regulation within the nucleus tractus solitarii.
基于细胞构筑学和免疫组织化学方法，猕猴孤束核被发现存在多个亚区。可识别的亚区包括连合部、内侧部、小细胞部、背外侧部、腹外侧部、中间部和间质部。采用过氧化物酶-抗过氧化物酶技术，P物质和脑啡肽免疫反应性定位于孤束核的离散区域。P物质免疫反应性在孤束核的间质亚区最为常见。在连合部、内侧部、小细胞部、背外侧部和中间亚区存在中等程度的P物质免疫反应性积聚，但在腹外侧亚区含量极少。脑啡肽免疫反应性遵循P物质的染色模式；然而，脑啡肽免疫反应性的量少于P物质。秋水仙碱处理后，大量脑啡肽免疫反应性神经元分布于所有亚区，许多位于小细胞部和内侧部。发现的少数P物质免疫反应性神经元局限于小细胞部。猕猴中P物质和脑啡肽免疫反应性的分布与猫和大鼠中描述的非常相似。此外，这两种假定神经递质分布的广泛重叠为它们可能参与孤束核内的自主调节提供了形态学证据。
