Sariguzel Fatma Mutlu, Kayman Tuba, Karaman Hatice, Karaman Ahmet, Karakukcu Cigdem
Department of Microbiology, Kayseri Education and Research Hospital, Kayseri, Turkey.
Department of Pathology, Kayseri Education and Research Hospital, Kayseri, Turkey.
Clin Lab. 2013;59(11-12):1403-8. doi: 10.7754/clin.lab.2013.121117.
Hepatitis C virus (HCV) is an important cause of chronic liver disease. There are six genotypes and more than 80 subtypes of HCV. The aim of this study was to investigate the distribution of HCV genotypes in Middle Anatolia in Turkey, and the association of HCV genotypes with pre-treatment HCV RNA viral load, serum transaminase levels, and histopathological grade of liver fibrosis.
A total of 160 patients (103 female, 57 male) with chronic hepatitis C were retrospectively evaluated. HCV RNA level was determined by commercial real time PCR method. HCV RNA positive sera were genotyped by the Abbott Real Time HCV Genotype II assay and sequenced by the ABI Prism 310 Genetic Analyzer. Gender, age, serum ALT, AST, HCV RNA viral load, and fibrosis staging of liver were determined in all patients.
Genotype 1b was the most frequent (64.7%) followed by genotype 4d (28.3%), 2 (4.4%), and la (2.5%). The HCV genotype results were found consistent with both methods. The gender distribution of the 160 HCV infected patients was 57 male/103 female. Log HCVRNA was significantly higher in genotype 1b compared to genotype 4 and 1a. Stage of liver fibrosis, histology activity index, serum ALT and AST levels did not differ between groups depending on genotypes. Advanced liver fibrosis (Group 2) was found in 36 (76.6%) patients with genotype 1b and in 10 (21.3%) patients with genotype 4 and only in 1 patient (2.1%) with genotype 1a.
HCV genotype 1b is the most frequent type (64.7%) in this region. Prevalence of genotype 4 in this region is higher than the national HCV genotype distribution. Serum transaminase levels and liver fibrosis scores are not associated with HCV genotypes.
丙型肝炎病毒（HCV）是慢性肝病的重要病因。HCV有六种基因型和80多种亚型。本研究的目的是调查土耳其安纳托利亚中部地区HCV基因型的分布情况，以及HCV基因型与治疗前HCV RNA病毒载量、血清转氨酶水平和肝纤维化组织病理学分级之间的关联。
对160例慢性丙型肝炎患者（103例女性，57例男性）进行回顾性评估。采用商业实时PCR法测定HCV RNA水平。HCV RNA阳性血清通过雅培实时HCV基因型II检测法进行基因分型，并通过ABI Prism 310遗传分析仪进行测序。测定所有患者的性别、年龄、血清ALT、AST、HCV RNA病毒载量以及肝脏纤维化分期。
1b基因型最为常见（64.7%），其次是4d基因型（28.3%）、2基因型（4.4%）和1a基因型（2.5%）。两种方法得出的HCV基因型结果一致。160例HCV感染患者的性别分布为57例男性/103例女性。与4基因型和1a基因型相比，1b基因型的Log HCVRNA显著更高。不同基因型组之间的肝纤维化分期、组织学活动指数、血清ALT和AST水平无差异。1b基因型的36例（76.6%）患者、4基因型的10例（21.3%）患者以及仅1a基因型的1例（2.1%）患者出现了晚期肝纤维化（2组）。
HCV 1b基因型是该地区最常见的类型（64.7%）。该地区4基因型的流行率高于全国HCV基因型分布情况。血清转氨酶水平和肝纤维化评分与HCV基因型无关。
