Liu Bin-yu, Liu Bin-yan, Li Li-fen, He Yin-fei, Wang Ya-rong
Medical College, Shanxi Datong University, Datong 037009, China.
Zhonghua Zheng Xing Wai Ke Za Zhi. 2013 Sep;29(5):370-5.
To observe the effect of zi-hua burn cream on the survival of skin flaps in rats, and its mechanisms.
72 Wistar rats, were randomly divided into four groups as zi-hua group(n = 18, external application of alfalfa burn cream), control group (n = 18, external application of heparin sodium cream), model group (n = 18, external application of vaseline) , negative control (n = 18, no operation). 8 cm x 2 cm random skin flaps with pedicle on the side of head were designed on the back of Wistar rats. The drug was applied on the flap surface, 2 times a day. The survival of skin flaps was observed. The change of serum superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO), turner necrosis factor-alpha(TNF-alpha)and interleukin-6(IL-6)were compared at 1,2,3,7 d after operation, and histologic examination was performed.
The survival rate of zi-hua group (73.58 - 10. 74)% was significantly higher than that of model group (33.40 - 16.05) %, showing a statistical difference (Q = 10.63, P <0.01). There was no significant difference between the zi-hua group and control group (71.65 +/- 11. 92) %. The level of serum SOD, NO in zi-hua group and control group was higher than that in model group, while the level of serum MDA, TNF-alpha and IL-6 was lower than that in model group(P <0.01). On 7 day after operation, skin flaps tissue edema,necrosis and inflammatory cell infiltration in zi-hua group and control group was less obvious than that in model group. There was significant proliferation of granuloma and fibroblast and formation of neonatal capillary in zi-hua group and control group. The vascular density in zi-hua group was obviously higher than that in the model group and control group(P <0. 01).
Zi-hua burn cream could significantly improve the blood supply of skin flaps, increase the survival rate of skin flaps in rats. Its mechanism may be associated with the anti-free-radical-damage action, improve local microcirculation, improve the NO content, reduce the TNF-alpha and IL-6 level, reduce inflammation factor release, improve oxidative stress state, and reduce inflammation reaction.
观察紫花烧伤膏对大鼠皮瓣存活的影响及其作用机制。
将72只Wistar大鼠随机分为四组，即紫花组（n = 18，外用紫花烧伤膏）、对照组（n = 18，外用肝素钠乳膏）、模型组（n = 18，外用凡士林）、阴性对照组（n = 18，未做手术）。在Wistar大鼠背部设计8 cm×2 cm带蒂随意型头部侧方皮瓣。将药物涂抹于皮瓣表面，每日2次。观察皮瓣存活情况。比较术后1、2、3、7 d血清超氧化物歧化酶（SOD）、丙二醛（MDA）、一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的变化，并进行组织学检查。
紫花组皮瓣存活率为（73.58 - 10.74）%，显著高于模型组的（33.40 - 16.05）%，差异有统计学意义（Q = 10.63，P <0.01）。紫花组与对照组（71.65±11.92）%比较，差异无统计学意义。紫花组和对照组血清SOD、NO水平高于模型组，而血清MDA、TNF-α和IL-6水平低于模型组（P <0.01）。术后7 d，紫花组和对照组皮瓣组织水肿、坏死及炎性细胞浸润较模型组轻。紫花组和对照组有明显的肉芽组织及成纤维细胞增生，并有新生毛细血管形成。紫花组血管密度明显高于模型组和对照组（P <０.01）。
紫花烧伤膏可显著改善皮瓣血供，提高大鼠皮瓣存活率。其机制可能与抗自由基损伤、改善局部微循环、提高NO含量、降低TNF-α和IL-6水平、减少炎症因子释放、改善氧化应激状态、减轻炎症反应有关。
