阐明羟基化和甲基化步骤定制皮里菌素 A1 的生物合成。
Elucidating hydroxylation and methylation steps tailoring piericidin A1 biosynthesis.
机构信息
Key Laboratory of Tropical Marine Bio-resources and Ecology, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences , 164 West Xingang Road, Guangzhou 510301, China.
出版信息
Org Lett. 2014 Feb 7;16(3):736-9. doi: 10.1021/ol4034176. Epub 2014 Jan 10.
The piericidin A1 (1) gene cluster was identified from the deep-sea derived Streptomyces sp. SCSIO 03032. Our in vivo and in vitro experiments verified PieE as a 4'-hydroxylase and PieB2 as a 4'-O-methyltransferase, allowing the elucidation of the post-PKS modification steps involved in 1 biosynthesis. In addition, the shunt metabolite piericidin E1 (7) was identified as a novel analogue featuring a C-2/C-3 epoxy ring.
从深海来源的链霉菌 SCSIO 03032 中鉴定出 piericidin A1（1）基因簇。我们的体内和体外实验验证了 PieE 是一个 4'-羟化酶，PieB2 是一个 4'-O-甲基转移酶，这使得 1 生物合成中涉及的聚酮化合物后修饰步骤得以阐明。此外，还鉴定出了分流代谢产物 piericidin E1（7），它是一种具有 C-2/C-3 环氧环的新型类似物。
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