鉴定青羊（Dama dama）胎盘的妊娠相关糖蛋白和甲胎蛋白。
Identification of pregnancy-associated glycoproteins and alpha-fetoprotein in fallow deer (Dama dama) placenta.
机构信息
Laboratory of Animal Endocrinology and Reproduction, Faculty of Veterinary Medicine, University of Liege, B-4000 Liege, Belgium.
出版信息
Acta Vet Scand. 2014 Jan 13;56(1):4. doi: 10.1186/1751-0147-56-4.
BACKGROUND
This paper describes the isolation and characterization of pregnancy-associated glycoproteins (PAG) from fetal cotyledonary tissue (FCT) and maternal caruncular tissue (MCT) collected from fallow deer (Dama dama) pregnant females. Proteins issued from FCT and MCT were submitted to affinity chromatographies by using Vicia villosa agarose (VVA) or anti-bovine PAG-2 (R#438) coupled to Sepharose 4B gel. Finally, they were characterized by SDS-PAGE and N-terminal microsequencing.
RESULTS
Four distinct fallow deer PAG (fdPAG) sequences were identified and submitted to Swiss-Prot database. Comparison of fdPAG with PAG sequences identified in other ruminant species exhibited 64 to 83% identity. Additionally, alpha-fetoprotein was identified in fetal and maternal tissues.
CONCLUSION
Our results demonstrate the efficacy of VVA and bovine PAG-2 affinity chromatographies for the isolation of PAG molecules expressed in deer placenta. This is the first report giving four specific amino acid sequences of PAG isolated from feto-maternal junction (FCT and MCT) in the Cervidae family.
背景
本研究从怀孕的赤鹿（Dama dama）胎儿绒毛膜组织（FCT）和母体胎盘组织（MCT）中分离和鉴定了妊娠相关糖蛋白（PAG）。使用金雀花凝集素琼脂糖（VVA）或与 Sepharose 4B 凝胶偶联的抗牛 PAG-2（R#438）对 FCT 和 MCT 来源的蛋白质进行亲和层析，然后通过 SDS-PAGE 和 N 端微测序进行鉴定。
结果
鉴定并提交了 4 种不同的赤鹿 PAG（fdPAG）序列至 Swiss-Prot 数据库。与其他反刍动物 PAG 序列的比较显示，fdPAG 具有 64%至 83%的同源性。此外，还在胎儿和母体组织中鉴定到了甲胎蛋白。
结论
我们的研究结果表明 VVA 和牛 PAG-2 亲和层析可有效分离鹿胎盘表达的 PAG 分子。这是首次从鹿科动物的胎-母体连接处（FCT 和 MCT）分离 PAG 并报告其 4 个特定的氨基酸序列。
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