Department of Biopathological Veterinary Science, Faculty of Veterinary Medicine, University of Perugia, Perugia, 06126, Italy.
BMC Vet Res. 2013 May 1;9:89. doi: 10.1186/1746-6148-9-89.
Pregnancy-associated glycoproteins (PAGs) were first described as placental antigens present in the blood serum of the mother soon after implantation. Here, we describe the purification of several pregnancy-associated glycoproteins from water buffalo placenta (wbPAGs). A specific radioimmunoassay (RIA) was developed for early pregnancy diagnosis in buffalo species.
Amino-terminal microsequencing of immunoreactive placental proteins allowed the identification of eleven wbPAGs sequences [Swiss-Prot accession numbers: P86369 to P86379]. Three polyclonal antisera (AS#858, AS#859 and AS#860) were raised in rabbits against distinct wbPAG fractions. A new RIA (RIA-860) was developed and used to distinguish between pregnant (n=33) and non-pregnant (n=26) water buffalo females.
Our results confirmed the multiplicity of PAG expression in buffalo placenta. In addition, the RIA-860 system was shown to be sensitive, linear, reproducible, accurate and specific in measuring PAG concentrations in buffalo plasma samples from Day 37 of gestation onwards.
妊娠相关糖蛋白（PAG）最初被描述为着床后不久母体血清中存在的胎盘抗原。在这里，我们描述了从水牛胎盘（wbPAG）中纯化几种妊娠相关糖蛋白的方法。我们开发了一种用于水牛物种早期妊娠诊断的特异性放射免疫分析（RIA）。
免疫反应性胎盘蛋白的氨基末端微测序允许鉴定十一种 wbPAG 序列[Swiss-Prot 登录号：P86369 至 P86379]。针对不同的 wbPAG 馏分，我们在兔子中产生了三种多克隆抗血清（AS#858、AS#859 和 AS#860）。开发了一种新的 RIA（RIA-860），用于区分怀孕（n=33）和未怀孕（n=26）的水牛雌性。
我们的结果证实了 PAG 在水牛胎盘中的多重表达。此外，RIA-860 系统在测量妊娠 37 天及以后的水牛血浆样品中的 PAG 浓度时表现出敏感、线性、可重复、准确和特异性。
