Department of Clinical Laboratory, The Second Xiangya Hospital, Central South University, Number 139 Middle Renming Road, Changsha 410011, People's Republic of China.
Department of Clinical Laboratory, Chenzhou Number 1 People's Hospital, Chenzhou, Hunan, People's Republic of China.
J Clin Neurosci. 2014 Mar;21(3):482-6. doi: 10.1016/j.jocn.2013.08.020. Epub 2013 Oct 30.
We investigated the activation and pathophysiological roles of indoleamine 2,3-dioxygenase (IDO) and kynurenine aminotransferase (KAT) in patients with ischemic stroke. Patients were recruited from the acute stroke unit of a general hospital within 24 hours post-stroke. The immune transmission turbidity method was used to determine the concentration of serum high-sensitivity C-reactive protein (hsCRP), apolipoprotein A-1 and apolipoprotein B. The concentrations of triglyceride, cholesterol, high density lipoprotein (HDL), low density lipoprotein and non-esterified fatty acids were determined using an enzymatic method. Tryptophan (TRP), kynurenine (KYN) and kynurenine acid (KYNA) concentrations were determined by high performance liquid chromatography. The National Institutes of Health Stroke Scale (NIHSS) was used to assess the neurological deficits at admission and 3 weeks post-stroke. The IDO and KAT activity ratio were calculated by KYN/TRP and KYNA/KYN, respectively. The correlation between hsCRP and IDO, KAT and NIHSS score was also analyzed. A total of 81 patients with ischemic stroke and 35 normal controls were recruited. Lower TRP, KYNA, HDL and KAT activity ratio were found in the stroke group compared to the control group (p<0.05). The levels of hsCRP and IDO activity ratio were much higher in the stroke group than the control group (p<0.01). The IDO activity in patients with ischemic stroke showed a positive correlation with hsCRP (r=0.425, p=0.027). In addition, hsCRP and IDO levels were positively associated with the NIHSS score both at admission and 3 weeks post-stroke. These data suggest an inflammatory response characterized by up-regulated IDO activation in ischemic stroke, which might be closely relevant to its pathophysiology.
我们研究了色氨酸 2,3-双加氧酶（IDO）和犬尿氨酸氨基转移酶（KAT）在缺血性脑卒中患者中的激活和病理生理作用。患者在卒中后 24 小时内从综合医院的急性卒中病房招募。采用免疫透射比浊法测定血清高敏 C 反应蛋白（hsCRP）、载脂蛋白 A-1 和载脂蛋白 B 的浓度。采用酶法测定甘油三酯、胆固醇、高密度脂蛋白（HDL）、低密度脂蛋白和非酯化脂肪酸的浓度。采用高效液相色谱法测定色氨酸（TRP）、犬尿氨酸（KYN）和犬尿氨酸酸（KYNA）的浓度。采用国立卫生研究院卒中量表（NIHSS）评估入院时和卒中后 3 周的神经功能缺损。通过 KYN/TRP 和 KYNA/KYN 分别计算 IDO 和 KAT 的活性比。还分析了 hsCRP 与 IDO、KAT 和 NIHSS 评分之间的相关性。共纳入 81 例缺血性卒中患者和 35 例正常对照者。与对照组相比，卒中组的 TRP、KYNA、HDL 和 KAT 活性比均降低（p<0.05）。卒中组的 hsCRP 和 IDO 活性比明显高于对照组（p<0.01）。缺血性卒中患者的 IDO 活性与 hsCRP 呈正相关（r=0.425，p=0.027）。此外，hsCRP 和 IDO 水平与入院时和卒中后 3 周时的 NIHSS 评分均呈正相关。这些数据表明，缺血性卒中存在以 IDO 激活上调为特征的炎症反应，这可能与其病理生理学密切相关。
