Colle J H, Perret R, Truffa-Bachi P
Mol Immunol. 1987 Jan;24(1):39-46. doi: 10.1016/0161-5890(87)90109-x.
Two anti-TNP antibodies exhibiting unusual features are described. They were obtained in two independent fusions. Spleen cells from CB20 mice sensitized with TNP-Ficoll and challenged with TNP-LPS were fused with SP2/0 myeloma cells. One of these hybridomas, CBT3, secretes antibodies which react with both monospecific anti-gamma 2b and anti-gamma 3 anti-isotypic sera; the second hybridoma, CBT4, secretes antibodies reacting with monospecific anti-mu and anti-gamma 2b sera. Only one type of immunoglobulin is secreted by each hybridoma, ruling out the hypothesis of hybrid molecules formed by distinct heavy chains. These results imply that the two heavy chains are made up from elements encoded by gamma 3 and gamma 2b genes in CBT3 and by gamma 2b and mu genes in CBT4. The molecular mechanisms underlying the production of these singular heavy chains are discussed.
描述了两种具有异常特征的抗三硝基苯（TNP）抗体。它们是在两次独立的融合实验中获得的。用TNP-菲可（Ficoll）致敏并用TNP-脂多糖（LPS）攻击的CB20小鼠的脾细胞与SP2/0骨髓瘤细胞进行融合。其中一种杂交瘤CBT3分泌的抗体可与单特异性抗γ2b和抗γ3同种型血清发生反应；第二种杂交瘤CBT4分泌的抗体可与单特异性抗μ和抗γ2b血清发生反应。每个杂交瘤仅分泌一种类型的免疫球蛋白，排除了由不同重链形成杂交分子的假设。这些结果表明，在CBT3中两条重链由γ3和γ2b基因编码的元件组成，而在CBT4中由γ2b和μ基因编码的元件组成。讨论了产生这些奇异重链的分子机制。
