Olson Joelle F, Moon Roger D, Kells Stephen A, Mesce Karen A
Department of Entomology, University of Minnesota, 219 Hodson Hall, 1980 Folwell Avenue, Saint Paul, MN 55108, United States.
Arthropod Struct Dev. 2014 Mar;43(2):117-22. doi: 10.1016/j.asd.2013.12.004. Epub 2014 Jan 8.
After blood feeding on a host, bed bugs, Cimex lectularius, assemble in aggregation sites away from the host. Off-host aggregation is mediated by a combination of mechanical and chemical stimuli associated with bug feces. Partial antennectomies indicated removal of flagellomeres did not affect aggregation, but removal of the whole pedicel or its distal half significantly reduced (P < 0.01) aggregation, suggesting that sensilla related to off-host aggregation occur on the distal half of the pedicel. Scanning electron microscopy (SEM) revealed that serrated hairs were distributed throughout the pedicel, but newly described smooth hairs were present mainly on the distal half, and a distinct patch of grooved pegs, smooth pegs and immersed cones was present on the posterior edge of the distal half of the pedicel in adults, but not in nymphs. Numbers of different types of sensilla increased significantly during metamorphosis from first instar to adult (P < 0.05), but were similar between genders (P = 0.11) and between females from a laboratory and field strain of bugs (P = 0.19). Transmission electron microscopy (TEM) revealed that cuticular pores were present in the two types of pegs, indicating that the pegs have an olfactory function. The smooth hairs resembled gustatory sensilla previously described in Cimex hemipterus F. The existence of both olfactory and gustatory sensilla on the distal half of the pedicel suggests those sensilla may be the sensory basis of off-host aggregation behavior.
在吸食宿主血液后，温带臭虫（Cimex lectularius）会聚集在远离宿主的聚集地点。离开宿主后的聚集是由与臭虫粪便相关的机械和化学刺激共同介导的。部分触角切除表明，去除鞭节并不影响聚集，但去除整个梗节或其远端一半会显著降低（P < 0.01）聚集，这表明与离开宿主后的聚集相关的感器位于梗节的远端一半。扫描电子显微镜（SEM）显示，锯齿状毛分布在整个梗节上，但新描述的光滑毛主要存在于远端一半，并且在成虫梗节远端一半的后缘有一片明显的带槽栓、光滑栓和凹陷锥，但若虫中没有。从一龄若虫到成虫的变态过程中，不同类型感器的数量显著增加（P < 0.05），但性别之间（P = 0.11）以及实验室菌株和野外菌株的雌性之间（P = 0.19）数量相似。透射电子显微镜（TEM）显示，两种类型的栓中都存在表皮孔，表明这些栓具有嗅觉功能。光滑毛类似于先前在热带臭虫（Cimex hemipterus F.）中描述的味觉感器。梗节远端一半同时存在嗅觉和味觉感器表明，这些感器可能是离开宿主后聚集行为的感觉基础。
