Olson J F, Moon R D, Kells S A
Department of Entomology, University of Minnesota, St. Paul, MN 55108, USA.
J Insect Physiol. 2009 Jun;55(6):580-7. doi: 10.1016/j.jinsphys.2009.03.001.
The bed bug, Cimex lectularius, aggregates under filter paper disks previously stained by adults. A multiple choice assay was used to determine differences in aggregation behavior among two strains, multiple lifestages, and levels of starvation. There were no differences in level of aggregation between established and recently derived strains, or among adults and nymphs of different instars. Propensity to aggregate decreased with time since feeding, but preference for stained disks remained high. We also examined which sensory structures mediate aggregation, and whether antennectomy affected movement, orientation, and arrestment under stained disks. Bed bugs that were left intact, blinded, or surgically altered by the removal of probosci or the distal antennal segments exhibited high levels of aggregation under stained disks. However, the removal of the pedicel significantly reduced aggregation compared to intact bugs. Video recordings of movement and orientation by bugs with intact, partial and complete antennectomies demonstrated that neither partial nor complete antennectomies affected walking speed, path straightness, direction of movement or frequency of encounters with either stained or clean disks. However, complete removal of both antennae significantly reduced the percentage of encounters with stained disks that resulted in arrestment. These findings suggest aggregation by bed bugs is a result of arrestment mediated by direct, close-range contact between sensilla on the pedicel and stained disks.
臭虫（温带臭虫）会聚集在先前被成虫染色的滤纸圆盘下。采用多项选择试验来确定两个品系、多个发育阶段以及饥饿程度之间聚集行为的差异。已建立的品系和最近衍生的品系之间，或不同龄期的成虫和若虫之间，聚集程度没有差异。聚集倾向会随着进食后的时间而降低，但对染色圆盘的偏好仍然很高。我们还研究了哪些感觉结构介导聚集，以及切除触角是否会影响在染色圆盘下的移动、定向和停留。保持完整、致盲或通过切除喙或触角远端节段进行手术改变的臭虫在染色圆盘下表现出高度聚集。然而，与完整的臭虫相比，切除梗节显著降低了聚集程度。对具有完整、部分和完全切除触角的臭虫的移动和定向进行视频记录表明，部分或完全切除触角均不影响行走速度、路径直线度、移动方向或与染色或清洁圆盘相遇的频率。然而，完全切除两根触角会显著降低与染色圆盘相遇后停留的百分比。这些发现表明，臭虫的聚集是由梗节上的感器与染色圆盘之间直接的近距离接触介导的停留行为的结果。
