胃泌素对分离的胃黏膜细胞中蛋白质合成的诱导作用。

Gastrin induction of protein synthesis in isolated gastric mucosal cells.

作者信息

Majumdar A N, Edgerton E A

出版信息

Proc Soc Exp Biol Med. 1987 Sep;185(4):396-402. doi: 10.3181/00379727-185-42560.

Abstract

Exposure of isolated rat gastric mucosal cells to 10(-10) and 10(-9) M gastrin (G-17-I) for 2 hr significantly stimulated [3H]leucine incorporation (15 min pulse) into protein by 100 and 212%, respectively, when compared with the basal levels. Doses beyond 10(-9) M lowered the maximal stimulatory effect of the hormone. Gastrin (10(-9) M) specifically stimulated the synthesis of five proteins in isolated gastric mucosal cells with apparent molecular weights of 105, 76, 71, 63, and 54 kDa. Actinomycin-D (10 micrograms/ml) completely abolished the gastrin-mediated stimulation of protein synthesis in isolated gastric mucosal cells.

摘要

将分离的大鼠胃黏膜细胞暴露于10⁻¹⁰ M和10⁻⁹ M胃泌素（G-17-I）中2小时，与基础水平相比，[³H]亮氨酸掺入蛋白质（15分钟脉冲）分别显著增加了100%和212%。超过10⁻⁹ M的剂量降低了该激素的最大刺激作用。胃泌素（10⁻⁹ M）特异性刺激分离的胃黏膜细胞中5种蛋白质的合成，其表观分子量分别为105、76、71、63和54 kDa。放线菌素-D（10微克/毫升）完全消除了胃泌素介导的分离胃黏膜细胞中蛋白质合成的刺激作用。