Department of Botany, Faculty of Science, University of Tokyo, Hongo, 113, Tokyo, Japan.
Planta. 1978 Jan;140(1):15-8. doi: 10.1007/BF00389374.
Cell morphogenesis in Closterium acerosum (Schrank) Ehrenberg was greatly influenced by colchicine. Addition of colchicine to the medium led to production of "tadpole-shaped" cells, by decreasing the length and increasing the thickness of the new semicells. Transversely oriented wall microtubules and microfibrils, characteristic of normally elongating semicells, were not observed in colchicine-treated semicells, randomly oriented microfibrils being present instead. About 3.5 h after septum formation, the randomly oriented microfibrils began to be overlaid by bundles of microfibrils as seen in normal semicells at the later stage of elongation. When colchicine treatment was terminated 1 h after septum formation, cell elongation was partially restored and microfibrils were deposited parallel to each other and transversely to the cell axis, indicating that the effect of colchicine on microfibril arrangement in growing semicells is reversible.
壁细胞形态发生在肋刺藻（Schrank）埃伦贝格中受到秋水仙碱的极大影响。向培养基中添加秋水仙碱会导致“蝌蚪形”细胞的产生，这是通过减少新半细胞的长度和增加其厚度来实现的。在秋水仙碱处理的半细胞中未观察到特征性的正常伸长半细胞的横向取向的壁微管和微纤维，而是存在随机取向的微纤维。在隔膜形成后约 3.5 小时，随机取向的微纤维开始被微纤维束覆盖，就像在伸长后期的正常半细胞中一样。当在隔膜形成后 1 小时终止秋水仙碱处理时，细胞伸长部分得到恢复，微纤维彼此平行且横向沉积在细胞轴上，表明秋水仙碱对生长中的半细胞中微纤维排列的影响是可逆的。
