Tjernlund U, Scheynius A
Scand J Immunol. 1987 Jul;26(1):1-6. doi: 10.1111/j.1365-3083.1987.tb02227.x.
The biological importance of the presence of class II transplantation antigens on highly differentiated epithelial cells such as keratinocytes in certain conditions, is still unknown. We have therefore investigated the antigen-presenting capacity of separated human epidermal cells obtained from tuberculin-reactive skin 6 days after intradermal injection of purified protein derivative (PPD). Earlier studies have shown a high percentage of HLA-DR-expressing keratinocytes at this time. Peripheral adherent blood cells were used as control stimulator cells and highly purified peripheral blood T lymphocytes as responder cells. The T-cell proliferation in response to PPD in the presence of autologous epidermal cells from normal and tuberculin-reactive skin was measured by [3H]thymidine incorporation on day 6. The latter cell population, 76-86% of which consisted of HLA-DR-expressing cells as judged by immunocytochemistry, induced a greater T-cell response to PPD than do normal epidermal cells. This discrepancy in the T-cell proliferation could not be explained by a difference in the numbers of anti-Leu 6 or anti-HLA-DQ-reactive Langerhans cells. The present data indicate that epidermal cell suspensions containing HLA-DR-expressing keratinocytes induce a greater T-cell response to PPD than do normal epidermal cells.
在某些情况下，高度分化的上皮细胞（如角质形成细胞）上存在II类移植抗原的生物学重要性仍然未知。因此，我们研究了在皮内注射纯化蛋白衍生物（PPD）6天后从结核菌素反应性皮肤中分离出的人表皮细胞的抗原呈递能力。早期研究表明，此时表达HLA - DR的角质形成细胞比例很高。外周贴壁血细胞用作对照刺激细胞，高度纯化的外周血T淋巴细胞用作反应细胞。在第6天通过[3H]胸苷掺入法测量在来自正常和结核菌素反应性皮肤的自体表皮细胞存在下，T细胞对PPD的增殖反应。通过免疫细胞化学判断，后一组细胞中76 - 86%由表达HLA - DR的细胞组成，其诱导的T细胞对PPD的反应比正常表皮细胞更强。T细胞增殖的这种差异不能用抗Leu 6或抗HLA - DQ反应性朗格汉斯细胞数量的差异来解释。目前的数据表明，含有表达HLA - DR的角质形成细胞的表皮细胞悬液比正常表皮细胞诱导更强的T细胞对PPD的反应。
