长春花悬浮细胞原生质体形成的细胞壁的组成。
Composition of the cell wall formed by protoplasts isolated from cell suspension cultures of Vinca rosea.
机构信息
Department of Botany, Faculty of Science, University of Tokyo, Hongo, 113, Tokyo, Japan.
出版信息
Planta. 1978 Jan;140(3):227-32. doi: 10.1007/BF00390252.
The biochemistry of cell-wall regeneration in protoplasts obtained from Vinca rosea L. (Catharanthus roseus (L.) G. Don) cells grown in suspension culture by isolating the regenerated wall and the extracellular polysaccharides of protoplasts cultured for various periods, and investigating their composition. Gas-liquid chromatography and tracer studies with D-[U-(14)C]glucose showed that the sugar composition of the extracellular polysaccharides was similar to that of the original cell culture, consisting mainly of polyuronide and 3,6-linked arabinogalactan. the regenerated cell wall was composed of non-cellulosic glucans having 1,3- and 1,4-linkages, while its content in pectic and hemicellulosic components was very low.
长春花悬浮培养细胞原生质体细胞壁再生的生物化学 通过分离再生细胞壁和培养不同时间的原生质体的细胞外多糖来研究长春花（长春花（L.）G. Don）细胞悬浮培养中获得的原生质体的细胞壁再生的生物化学。用 D-[U-（14）C]葡萄糖进行的气液色谱和示踪研究表明，细胞外多糖的糖组成与原始细胞培养物相似，主要由聚半乳糖醛酸和 3,6 连接的阿拉伯半乳聚糖组成。再生细胞壁由具有 1,3-和 1,4-键的非纤维素葡聚糖组成，而其在果胶和半纤维素成分中的含量非常低。
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