John Innes Institute, Colney Lane, NR4 7UH, Norwich, U.K..
Planta. 1976 Jan;131(2):173-8. doi: 10.1007/BF00389991.
The process of cell wall regeneration around two species of higher plant protoplasts has been studied using reflection scanning electron microscopy. The first stage in the process is the formation of short fibres from randomly spaced centres. With protoplasts of tobacco leaf (Nicotiana tabacum L., cv White Burley) these fibres then elongate and interlace apparently at random to give rise to a matted continuous layer of wall. Protoplasts of a suspension culture of grapevine cells (Vitis vinifera L. cv Müller Thurgau) produce short fibres but these fail to elongate. Budding is observed during wall regeneration around vine protoplasts. The results are discussed in terms of the mechanical properties of the wall and its relationship to changes in plasmalemma morphology which are observed during wall formation.
使用反射扫描电子显微镜研究了两种高等植物原生质体细胞壁再生的过程。该过程的第一步是在随机间隔的中心形成短纤维。对于烟草叶（Nicotiana tabacum L.，cv White Burley）原生质体，这些纤维随后伸长并随机交织，形成一个交织的连续壁层。悬浮培养的葡萄细胞（Vitis vinifera L.，cv Müller Thurgau）原生质体产生短纤维，但这些纤维无法伸长。在葡萄原生质体周围的细胞壁再生过程中观察到出芽。根据细胞壁的机械性能及其与形成细胞壁过程中观察到的质膜形态变化的关系讨论了这些结果。
