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光需求、光敏色素和开花诱导素对 Pharbits nil Chois 的影响:二、对光敏色素转化的分光光度分析方法的批判性考察。

Light requirement, phytochrome and photoperiodic induction of flowering of Pharbits nil Chois : II. A critical examination of spectrophotometric assays of phytochrome transformations.

机构信息

Division of Plant Industry, CSIRO, P.O. Box 1600, 3601, Canberra City, A.C.T., Australia.

出版信息

Planta. 1978 Jan;141(1):9-14. doi: 10.1007/BF00387737.

Abstract

The low chlorophyll content of cotyledons of Pharbitis nil grown for 24 h in far-red light (FR) or at 18° C in white light from fluorescent lamps (WL) allows spectrophotometric measurement of phytochrome in these tissues. The Δ(ΔA) measurements utilize measuring beams at 730/802 nm and an actinic irradiation in excess of 90 s. The constancy of the relationship between phytochrome content and sample thickness confirms that, under these conditions of measurement, a true maximum phytochrome signal was obtained. These techniques have been used to follow changes in the form and amount of phytochrome during an inductive dark period for flowering. Following exposure to 24h WL at 18° C with a terminal 10 min red (R), Pfr was lost rapidly in darkness and approached zero in less than 1 h; during this period there was no change in the total phytochrome signal. Following exposure to 24 h FR with a terminal 10 min R, Pfr approached zero in 3 h, and the total phytochrome signal decreased by about half. The relevance of these changes to photoperiodic time measurement is discussed.

摘要

在远红光(FR)中培养 24 小时或在荧光灯下的白光(WL)中于 18°C 下生长的菟丝子子叶叶绿素含量低,允许在这些组织中分光光度法测量光敏素。Δ(ΔA)测量使用 730/802nm 的测量光束和超过 90s 的光激活照射。在测量条件下,样品厚度与光敏素含量之间的关系恒定,证实获得了真正的最大光敏素信号。这些技术已用于在诱导的黑暗开花期期间跟踪光敏素的形式和数量的变化。在 18°C 的 24 小时 WL 暴露后,用 10 分钟的红色(R)进行末端照射,Pfr 在黑暗中迅速丧失,在不到 1 小时内接近零; 在这段时间内,总光敏素信号没有变化。在 24 小时 FR 暴露后,用 10 分钟的红色(R)进行末端照射,Pfr 在 3 小时内接近零,总光敏素信号下降约一半。讨论了这些变化对光周期时间测量的相关性。

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