叶绿素对光敏色素测量的干扰。
Chlorophyll interference with phytochrome measurement.
机构信息
Department of Botany Plant Science Laboratories, University of Reading, Whiteknights, RG6 2AS, Reading, UK.
出版信息
Planta. 1977 Jan;135(2):119-23. doi: 10.1007/BF00387159.
Measurements of phytochrome by Δ (ΔA725-815 nm) were completely suppressed at chlorophyll concentrations of the order of 20-40 μg g(-1) f.wt. in vivo and 37 μg cm(-3) in vitro, and the readings were reduced by 50% at only 12 μg cm(-3) in vitro. At these concentrations of chlorophyll in aqueous methanol, the loss of phytochrome signal in vitro appeared to be due to failure of phytochrome photoconversion rather than to interference with ΔA measuremebt by chlorophyll fluorescence in the 125/815 nm measuring beam.
在体内的叶绿素浓度约为 20-40μg g(-1)f.wt. 和体外的 37μg cm(-3)时，用 Δ (ΔA725-815nm) 测量的光敏色素完全被抑制，而在体外仅 12μg cm(-3)时，读数就降低了 50%。在甲醇水溶液中，这些叶绿素浓度下，体外光敏色素信号的损失似乎是由于光敏色素光转化失败，而不是由于在 125/815nm 测量光束中叶绿素荧光对 ΔA 测量的干扰。
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