金黄色葡萄球菌小型细菌素生成质粒之间的不相容性和分子关系。

Incompatibility and molecular relationships between small bacteriocinogenic plasmids of Staphylococcus aureus.

机构信息

Departamento de Microbiologia Geral, Instituto de Microbiologia da UFRJ, CCS, Bloco I, Cidade Universitária, 21944-970, Rio de Janeiro, RJ, Brazil.

出版信息

World J Microbiol Biotechnol. 1995 Sep;11(5):525-8. doi: 10.1007/BF00286367.

DOI:10.1007/BF00286367

PMID:24414907

Abstract

The sequence relations between small bacteriocinogenic plasmids (pRJ6, pRJ9, pRJ10 and pRJ11) of Staphylococcus aureus were investigated by comparing restriction maps and by hybridization. Plasmids pRJ10 and pRJ11 showed identical restriction maps, similar to that of pRJ9. The restriction map of pRJ6 differed from those of pRJ9 and pRJ10/pRJ11. Both groups of plasmids were shown to share a region of homology of at least 2.6 kb. The incompatibility relationships between them were also investigated by using plasmid derivatives tagged with transposon Tn551. Plasmids pRJ6 and pRJ9 proved to belong to different incompatibility groups.

摘要

通过比较限制图谱和杂交，研究了金黄色葡萄球菌小型细菌素生成质粒（pRJ6、pRJ9、pRJ10 和 pRJ11）之间的序列关系。质粒 pRJ10 和 pRJ11 显示出相同的限制图谱，类似于 pRJ9。pRJ6 的限制图谱与 pRJ9 和 pRJ10/pRJ11 不同。这两组质粒显示出至少 2.6 kb 的同源区域。还使用带有转座子 Tn551 的质粒衍生物研究了它们之间的不相容性关系。质粒 pRJ6 和 pRJ9 被证明属于不同的不相容性群。