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原卟啉(Ⅸ)和叶绿素(Ⅸ)在黄化和绿化绿豆叶片中的磷光:谱带归属。

Phosphorescence of protochlorophyll(ide) and chlorophyll(ide) in etiolated and greening bean leaves : Assignment of spectral bands.

机构信息

A.N. Bakh Institute of Biochemistry, Academy of Sciences of the USSR, 117071, Moscow, USSR.

出版信息

Photosynth Res. 1991 Oct;30(1):7-14. doi: 10.1007/BF00035677.

Abstract

The assignment is presented for the principal phosphorescence bands of protochlorophyll(ide), chlorophyllide and chlorophyll in etiolated and greening bean leaves measured at -196°C using a mechanical phosphoroscope. Protochlorophyll(ide) phosophorescence spectra in etiolated leaves consist of three bands with maxima at 870, 920 and 970 nm. Excitation spectra show that the 870 nm band belongs to the short wavelength protochlorophyll(ide), P627. The latter two bands correspond to the protochlorophyll(ide) forms, P637 and P650. The overall quantum yield for P650 phosphorescence in etiolated leaves is near to that in solutions of monomeric protochlorophyll, indicating a rather high efficiency of the protochlorophyll(ide) triplet state formation in frozen plant material. Short-term (2-20 min) illumination of etiolated leaves at the temperature range from -30 to 20°C leads to the appearance of new phosphorescence bands at about 990-1000 and 940 nm. Judging from excitation and emission spectra, the former band belongs to aggregated chlorophyllide, the latter one, to monomeric chlorophyll or chlorophyllide. This indicates that both monomeric and aggregated pigments are formed at this stage of leaf greening. After preillumination for 1 h at room temperature, chlorophyll phosphorescence predominates. The spectral maximum of this phosphorescence is at 955-960 nm, the lifetime is about 2 ms, and the maximum of the excitation spectrum lies at 668 nm. Further greening leads to a sharp drop of the chlorophyll phosphorescence intensity and to a shift of the phosphorescence maximum to 980 nm, while the phosphorescence lifetime and a maximum of the phosphorescence excitation spectrum remains unaltered. The data suggest that chlorophyll phosphorescence belongs to the short wavelength, newly synthesized chlorophyll, not bound to chloroplast carotenoids. Thus, the phosphorescence measurement can be efficiently used to study newly formed chlorophyll and its precursors in etiolated and greening leaves and to address various problems arising in the analysis of chlorophyll biosynthesis.

摘要

本文介绍了在-196°C 下使用机械磷光计测量绿豆幼苗和绿化叶片中原叶绿素(ide)、叶绿素ide 和叶绿素的主要磷光带。绿豆幼苗中原叶绿素(ide)的磷光光谱由三个带组成,最大波长分别在 870、920 和 970nm 处。激发光谱表明,870nm 带属于短波长原叶绿素(ide),P627。后两个带对应于原叶绿素(ide)形式,P637 和 P650。绿豆幼苗中原叶绿素(ide)P650 磷光的总量子产率接近于单体原叶绿素溶液中的量子产率,表明在冷冻植物材料中原叶绿素(ide)三重态的形成效率相当高。在-30 至 20°C 的温度范围内,对绿豆幼苗进行 2-20 分钟的短期光照,会在约 990-1000nm 和 940nm 处出现新的磷光带。从激发和发射光谱来看,前一个带属于聚集态叶绿素ide,后一个带属于单体叶绿素或叶绿素ide。这表明在叶片变绿的这个阶段,单体和聚集的色素都在形成。在室温下预照 1 小时后,叶绿素磷光占主导地位。这种磷光的光谱最大值在 955-960nm 之间,寿命约为 2ms,激发光谱的最大值位于 668nm。进一步的绿化导致叶绿素磷光强度急剧下降,磷光最大值移至 980nm,而磷光寿命和磷光激发光谱的最大值保持不变。这些数据表明,叶绿素磷光属于新合成的、与叶绿体类胡萝卜素结合较弱的短波长叶绿素。因此,磷光测量可有效地用于研究绿豆幼苗和绿化叶片中新生叶绿素及其前体,并解决叶绿素生物合成分析中出现的各种问题。

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