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World J Microbiol Biotechnol. 1996 May;12(3):213-6. doi: 10.1007/BF00360917.
Removal of non-covalently attached polysaccharides from carboxymethylcellulase (CMCase) of Aspergillus niger improved its activity but decreased its thermostability and protease resistance. The activation energy profile of the hydrolysis of carboxymethylcellulose (CMC) was triphasic with increasing values of 17,-55 and-562 kJ/mol for polysaccharide-free and 19, -21 and -207 kJ/mol for polysaccharide-complexed CMCase. The specificity constant (Vmax/Km) of polysaccharide-free CMCase was 1.41 compared to polysaccharide-complexed CMCase which was only 0.68. The polysaccharide free CMCase had lower thermostability ('melting point' = 82°C) and higher protease susceptibility compared to polysaccharide-complexed CMCase ('melting point'>100°C).
从黑曲霉羧甲基纤维素酶（CMCase）中去除非共价结合的多糖可提高其活性，但降低其热稳定性和蛋白酶抗性。羧甲基纤维素（CMC）水解的活化能谱呈三相变化，无多糖的 CMCase 的值分别为 17、-55 和-562 kJ/mol，多糖复合的 CMCase 的值分别为 19、-21 和-207 kJ/mol。无多糖的 CMCase 的比活性常数（Vmax/Km）为 1.41，而多糖复合的 CMCase 仅为 0.68。与多糖复合的 CMCase（“熔点”>100°C）相比，无多糖的 CMCase 的热稳定性较低（“熔点”=82°C），且对蛋白酶的敏感性更高。
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