Department of Neurology, The First Affiliated Hospital at Chongqing Medical University, Chongqing, China ; Chongqing Key Laboratory of Neurobiology, Chongqing, China ; Institute of Neuroscience, Chongqing Medical University, Chongqing, China.
Suzhou Xishan Zhongke Laboratory Animal Co., Ltd., Suzhou, China.
PLoS One. 2014 Jan 8;9(1):e84599. doi: 10.1371/journal.pone.0084599. eCollection 2014.
The cynomolgus monkey (Macaca fascicularis) has been increasingly used as a non-human primate model in biomedical research. As establishing baseline thoracic radiography for the cynomolgus monkey is essential, we tested the hypothesis that age and sex may affect the thoracic radiography parameters of this species.
Here, 697 healthy cynomolgus monkeys were segregated by sex and age (three age groups: 25-36 months, 37-48 months, 49-60 months). The lung length (LL), maximal interior thoracic depth (TD), maximal interior thoracic breadth (TBr), cardiac silhouette breadth (CBr), cardiothoracic ratio (CR), right and left costophrenic angles (RCA and LCA), and right hilar height ratio (R-HHR) were assessed by chest film. Statistical analysis was applied to examine the effect of age, sex, and age × sex interactions.
Significant effects by age were shown for LL, TD, TBr, CBr, and CR. Significant effects by sex were found for TD, TBr, CBr, CR, and R-HHR. Significant effects by age × sex were observed for TD, TBr, CBr, and CR. Both TD and TBr increased with age in both sexes, and both were significantly higher in males than in females in the group aged 49-60 months. CBr increased with age and was significantly higher in males than in females across all age groups. CR declined with age and was significantly higher in males than females across all age groups, and CR was similar or slightly higher relative to those previously found in other non-human primate species. As to the other parameters with no significant sex nor age-related differences, the R-HHR was greater than 1.00, and the angulation of bilateral costophrenic angles were sharp.
The thoracic radiographic parameters for the healthy cynomolgus monkey presented here should prove useful in veterinary practice, research involving non-human primate models of respiratory or cardiovascular disorders, and morphological studies on cynomolgus monkeys.
食蟹猴（Macaca fascicularis）已被越来越多地用作生物医学研究中的非人类灵长类动物模型。由于建立食蟹猴的基本胸部 X 射线摄影术至关重要，我们测试了年龄和性别可能影响该物种胸部 X 射线摄影参数的假设。
在这里，将 697 只健康的食蟹猴按照性别和年龄（三个年龄组：25-36 个月，37-48 个月，49-60 个月）进行分组。通过胸部 X 射线评估肺长度（LL）、最大胸内深度（TD）、最大胸内宽度（TBr）、心影宽度（CBr）、心胸比（CR）、右和左肋膈角（RCA 和 LCA）以及右肺门高度比（R-HHR）。应用统计学分析来检查年龄、性别以及年龄×性别相互作用的影响。
年龄对 LL、TD、TBr、CBr 和 CR 有显著影响。性别对 TD、TBr、CBr、CR 和 R-HHR 有显著影响。年龄×性别对 TD、TBr、CBr 和 CR 有显著影响。TD 和 TBr 在两个性别中均随年龄增长而增加，在 49-60 个月龄的雄性中均显著高于雌性。CBr 随年龄增长而增加，在所有年龄组中雄性均显著高于雌性。CR 随年龄下降，在所有年龄组中雄性均显著高于雌性，CR 与其他非人类灵长类动物物种的结果相似或略高。至于其他没有显著性别或年龄相关差异的参数，R-HHR 大于 1.00，双侧肋膈角的角度较锐。
本文提供的健康食蟹猴的胸部 X 射线摄影参数应在兽医实践、涉及呼吸或心血管疾病的非人类灵长类动物模型的研究以及食蟹猴的形态学研究中证明是有用的。
