Sewelam Nasser, Oshima Yoshimi, Mitsuda Nobutaka, Ohme-Takagi Masaru
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, 305-8566, Japan; Botany Department, Faculty of Science, Tanta University, 31527, Tanta, Egypt.
Plant Cell Environ. 2014 Sep;37(9):2024-35. doi: 10.1111/pce.12274. Epub 2014 Feb 20.
In natural habitats, especially in arid areas, plants are often simultaneously exposed to multiple abiotic stresses, such as salt, osmotic and heat stresses. However, most analyses of gene expression in stress responses examine individual stresses. In this report, we compare gene expression in individual and combined stresses. We show that combined stress treatments with salt, mannitol and heat induce a unique pattern of gene expression that is not a simple merge of the individual stress responses. Under multiple stress conditions, expression of most heat and salt stress-responsive genes increased to levels similar to or higher than those measured in single stress conditions, but osmotic stress-responsive genes increased to lower levels. Genes up-regulated to higher levels under multiple stress condition than single stress conditions include genes for heat shock proteins, heat shock regulators and late embryogenesis abundant proteins (LEAs), which protect other proteins from damage caused by stresses, suggesting their importance in multiple stress condition. Based on this analysis, we identify candidate genes for engineering crop plants tolerant to multiple stresses.
在自然栖息地，尤其是干旱地区，植物常常同时遭受多种非生物胁迫，如盐胁迫、渗透胁迫和热胁迫。然而，大多数关于胁迫响应中基因表达的分析都只研究单一胁迫。在本报告中，我们比较了单一胁迫和复合胁迫下的基因表达情况。我们发现，盐、甘露醇和热的复合胁迫处理会诱导出一种独特的基因表达模式，并非单个胁迫响应的简单叠加。在多重胁迫条件下，大多数热胁迫和盐胁迫响应基因的表达水平升高至与单一胁迫条件下相当或更高的水平，但渗透胁迫响应基因的表达水平升高幅度较小。在多重胁迫条件下上调至比单一胁迫条件更高水平的基因包括热休克蛋白基因、热休克调节因子基因和晚期胚胎发生丰富蛋白（LEA）基因，这些基因可保护其他蛋白质免受胁迫造成的损伤，表明它们在多重胁迫条件下具有重要作用。基于此分析，我们鉴定出了用于培育耐多重胁迫作物的候选基因。
