Sheedlo H J, Siegel G J
Brain Res. 1987 Jul 7;415(1):105-14. doi: 10.1016/0006-8993(87)90273-3.
Na+,K+ ATPase and myelin-associated glycoprotein (MAG) were studied by immunocytochemistry on paraffin sections of the spinal cord, optic nerve and trigeminal ganglion of adult control (+/+) and CNS myelin-deficient shiverer (shi/shi) mice. Immunostaining for Na+, K+-ATPase outlined the periphery of nerve fibers in the spinal cord white matter, optic nerve and trigeminal ganglion of +/+ and shi/shi mice. Immunostaining for Na+,K+-ATPase appeared somewhat denser in the optic nerve and spinal cord lateral funiculi of shi/shi than in +/+ mice. In addition, immunostaining for Na+,K+-ATPase was demonstrated at the plasmalemma of presumed satellite cells situated at the periphery of ganglion cell bodies in the trigeminal ganglion of both species of mice. Immunostaining for MAG was localized along the periphery of nerve fibers in the spinal cord funiculi (with little immunostaining within gray horns), optic nerve and trigeminal ganglion of both +/+ and shi/shi mice. The major differences between shi/shi and +/+ mice were that the number of MAG-immunostained nerve fibers was greatly reduced in the spinal cord funiculi and the density of immunostaining was slightly increased in the optic nerve of shi/shi mice. The numbers of MAG-immunostained nerve fibers in trigeminal ganglion were similar in both species. Also, the cytoplasm of some oligodendrocyte-like cells was found densely immunostained for MAG in the spinal cord and optic nerve of shi/shi mice, but not of +/+ mice. This light microscopic study provides evidence that the defective shiverer gene leads to a decrease in MAG deposition and to aggregations of MAG-like material within perikarya of oligodendrocyte-like cells in regions of the CNS.
通过免疫细胞化学方法，对成年对照（+/+）和中枢神经系统髓磷脂缺陷型颤抖小鼠（shi/shi）的脊髓、视神经和三叉神经节石蜡切片中的钠钾ATP酶（Na +,K + ATPase）和髓磷脂相关糖蛋白（MAG）进行了研究。在+/+和shi/shi小鼠的脊髓白质、视神经和三叉神经节中，钠钾ATP酶的免疫染色勾勒出神经纤维的外周轮廓。shi/shi小鼠视神经和脊髓外侧索中钠钾ATP酶的免疫染色比+/+小鼠略显浓密。此外，在两种小鼠三叉神经节中，位于神经节细胞体外周的假定卫星细胞的质膜上也显示出钠钾ATP酶的免疫染色。在+/+和shi/shi小鼠的脊髓白质（灰质角内免疫染色较少）、视神经和三叉神经节中，MAG的免疫染色定位于神经纤维外周。shi/shi和+/+小鼠之间的主要差异在于，shi/shi小鼠脊髓白质中MAG免疫染色的神经纤维数量大幅减少，视神经中免疫染色密度略有增加。两种小鼠三叉神经节中MAG免疫染色的神经纤维数量相似。此外，在shi/shi小鼠而非+/+小鼠的脊髓和视神经中，发现一些少突胶质细胞样细胞的细胞质中MAG免疫染色浓密。这项光学显微镜研究提供了证据，表明有缺陷的颤抖基因导致中枢神经系统区域内少突胶质细胞样细胞的核周体中MAG沉积减少和MAG样物质聚集。
