Muganda-Ojiaku P M, Huang E S
Cancer Biochem Biophys. 1987 May;9(2):179-89.
Human fibroblastic cell lines morphologically transformed by either live virus or DNA fragments of human cytomegalovirus had altered plasma membrane protein composition; quantitative changes, and gains and losses in protein composition in comparison to normal parent cell lines were detected. These transformed cell lines showed altered total cell protein phosphorylation patterns when compared to parent cell lines. A two to four fold increase in in vivo protein phosphorylation at serine and threonine residues was observed; no increase in phosphorylation at total cell tyrosine residues was detected. Analysis of the in vivo phosphorylated protein by two dimensional gel electrophoresis revealed some similarities as well as differences in the types of polypeptides phosphorylated between transformed and control cell lines. Increased (two-to sixfold over parent cell extracts) casein kinase and polyamine dependent casein kinase activities were detected in HCMV transformed cell extracts.
由人巨细胞病毒的活病毒或DNA片段进行形态转化的人成纤维细胞系，其质膜蛋白组成发生了改变；与正常亲代细胞系相比，检测到了蛋白质组成的定量变化以及增减情况。与亲代细胞系相比，这些转化细胞系的总细胞蛋白磷酸化模式发生了改变。观察到丝氨酸和苏氨酸残基的体内蛋白磷酸化增加了两到四倍；未检测到总细胞酪氨酸残基的磷酸化增加。通过二维凝胶电泳对体内磷酸化蛋白进行分析，发现转化细胞系和对照细胞系之间磷酸化多肽的类型既有一些相似之处，也有差异。在人巨细胞病毒转化细胞提取物中检测到酪蛋白激酶和多胺依赖性酪蛋白激酶活性增加（比亲代细胞提取物高两到六倍）。
