Department of Cellular Biology and Histology, IMIB, Medical School, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Murcia, Spain.
Reproductive Biology Unit, Department of Biology, Faculty of Sciences, University of Girona, Campus de Montilivi, Girona, Spain.
Theriogenology. 2014 Mar 15;81(5):702-11. doi: 10.1016/j.theriogenology.2013.12.004. Epub 2013 Dec 12.
It is important to understand the proliferative activity of the different structures of the male reproductive apparatus in livestock species, such as Sus domesticus, to ensure reproductive efficiency. The main aims of this study were (a) to evaluate the proliferative activity of the spermatogonia in the different stages of the seminiferous cycle and (b) to study the cell proliferation in the epididymal epithelium in each region, identifying the different cells involved. For this, the testes and epididymis of three healthy, sexually mature Sus domesticus boars were used. The organs were processed for light microscopy, and immunohistochemical techniques were used to detect proliferating cell nuclear antigen. The cells immunostaining positively and negatively for proliferating cell nuclear antigen were counted and several parameters and indexes were calculated to evaluate the proliferation in both epithelia, taking into account the stage of the seminiferous epithelium cycle, and, in the case of the epididymal epithelium, the different regions and cells are the same. Finally, a contrast analysis of equality between pairs of means was carried out followed by a least significant differences test, in which differences were considered significant at P < 0.05. In the seminiferous epithelium, the greatest total number of spermatogonia and proliferating spermatogonia was observed in the postmeiotic stages (mainly VII and VIII). The proliferation index of the spermatogonia increased from the meiotic to postmeiotic stages. As regards the epididymal epithelium, the total proliferation index was higher in the caput. In each region, the clear and principal cells showed the highest proliferation index with respect to the total number of cells counted, whereas the proliferation index of each cell with respect to the same type was higher in the clear cells, followed by the narrow and principal cells. In conclusion, the proliferative activity of spermatogonia in the seminiferous epithelium of Sus domesticus is stage-dependent, and mainly occurs in the postmeiotic stages. In the epididymal epithelium, proliferative activity takes place in several cell types and is dependent on the anatomical region of the epididymis. We think that these results may be of importance for understanding the pathologic or reproductive processes in which cell proliferation is involved in the male reproductive system.
了解家畜生殖器官不同结构的增殖活性很重要，例如Sus domesticus，以确保繁殖效率。本研究的主要目的是：（a）评估精原细胞在生精周期不同阶段的增殖活性；（b）研究每个区域附睾上皮的细胞增殖，并鉴定参与的不同细胞。为此，使用了 3 只健康、性成熟的 Sus domesticus 公猪的睾丸和附睾。对器官进行了光镜处理，并使用免疫组织化学技术检测增殖细胞核抗原。对增殖细胞核抗原呈阳性和阴性免疫染色的细胞进行计数，并计算了几个参数和指数，以评估两个上皮的增殖，考虑到生精上皮周期的阶段，并且在附睾上皮的情况下，不同区域和细胞相同。最后，进行了配对均值均等性的对比分析，然后进行了最小显著差异检验，其中 P < 0.05 时认为差异显著。在生精上皮中，在后减数分裂阶段（主要是 VII 和 VIII 期）观察到最多的精原细胞和增殖精原细胞总数。精原细胞的增殖指数从减数分裂到后减数分裂阶段增加。至于附睾上皮，头部的总增殖指数较高。在每个区域中，透明细胞和主细胞的总增殖指数最高，与计数的总细胞数相比，而对于每种细胞类型，透明细胞的增殖指数更高，其次是狭窄细胞和主细胞。总之，Sus domesticus 生精上皮中精原细胞的增殖活性与阶段有关，主要发生在后减数分裂阶段。在附睾上皮中，增殖活性发生在几种细胞类型中，并且取决于附睾的解剖区域。我们认为这些结果可能对于理解涉及雄性生殖系统中细胞增殖的病理或生殖过程很重要。
