Kelland L R, Burgess L, Steel G G
Cancer Res. 1987 Sep 15;47(18):4947-52.
Four continuous cell lines were established from 15 biopsies of human squamous carcinomas of the uterine cervix, two from women less than 35 years old. All four lines grew as adherent monolayers and had epitheloid morphology. All required initial 3T3 feeder layer support and hydrocortisone and insulin for growth and have now been grown in vitro for at least 12 months. The individual lines possessed unique isozyme patterns and were distinct from the HeLa cell line. All were tumorigenic in nude mice. In vitro colony forming efficiencies ranged from 2 to 30% in a monolayer anchorage dependent assay but were only from 0.0025 to 0.6% when assayed in soft agar. The lines were all aneuploid with mean chromosome numbers ranging from 71 to 75. Analysis of intermediate filament expression showed that all lines were positive for cytokeratin expression and two were positive for vimentin expression. These low-passage cell lines represent a panel of new in vitro models of carcinoma of the cervix. They should be useful for the investigation of chemosensitivity, of the involvement of human Papillomavirus in this disease, and as models of squamous cell differentiation.
从15例人宫颈鳞状癌活检组织中建立了4个连续细胞系，其中2个来自年龄小于35岁的女性。所有4个细胞系均以贴壁单层形式生长，具有上皮样形态。所有细胞系最初都需要3T3饲养层支持以及氢化可的松和胰岛素才能生长，目前已在体外培养至少12个月。各个细胞系具有独特的同工酶模式，且与HeLa细胞系不同。所有细胞系在裸鼠中均具有致瘤性。在单层锚定依赖性试验中，体外集落形成效率为2%至30%，但在软琼脂中检测时仅为0.0025%至0.6%。这些细胞系均为非整倍体，平均染色体数在71至75之间。中间丝表达分析表明，所有细胞系细胞角蛋白表达均为阳性，2个波形蛋白表达为阳性。这些低传代细胞系代表了一组新的宫颈癌体外模型。它们可用于化学敏感性研究、人乳头瘤病毒在该疾病中的作用研究以及作为鳞状细胞分化模型。
